Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration.
Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration.
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DOI：
10.1016/j.xpro.2021.100720
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Lin C;Liu Z;Li Y;Chen J
发热是一种复杂的生理反应，在感染和炎症期间增强免疫监视。发热范围全身热疗（WBH）治疗可以在实验上模拟小鼠的发热状况。在这里，我们描述了一个协议的治疗小鼠全身热休克和正常体温。我们描述了从小鼠脾脏中分离T细胞，然后评价T细胞粘附和迁移。该动物模型可用于研究发热引起的免疫功能紊乱。有关本方案使用和执行的完整详细信息，请参阅。全身热疗（whole body hyperthermia，WBH）可以模拟小鼠的发热状态。我们从WBH或常温处理的小鼠中分离T细胞，T细胞粘附和迁移试验显示发热引起的功能障碍。发热范围全身热疗（WBH）治疗可以在实验上模拟小鼠的发热状况。在这里，我们描述了一个协议的治疗小鼠全身热休克和正常体温。我们描述了从小鼠脾脏中分离T细胞，然后评价T细胞粘附和迁移。该动物模型可用于研究发热引起的免疫功能紊乱。
Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to. Whole-body hyperthermia (WBH) can mimic the febrile condition in mice We isolate T cells from WBH- or normothermia-treated mice T-cell adhesion and transmigration assays show dysfunctions caused by fever Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever.
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影响因子：
3.1
作者：
Evans, SS;Bain, MD;Wang, WC
通讯作者：
Wang, WC
DOI：
10.1038/nri3843
发表时间：
2015-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Evans SS;Repasky EA;Fisher DT
通讯作者：
Fisher DT
影响因子：
32.4
作者：
Chen, Q;Wang, WC;Evans, SS
通讯作者：
Evans, SS
影响因子：
20.3
作者：
Evans, SS;Wang, WC;Repasky, EA
通讯作者：
Repasky, EA
影响因子：
4.4
作者：
Ostberg, JR;Gellin, C;Repasky, EA
通讯作者：
Repasky, EA