Implantation, transplantation, and epithelial-mesenchymal relationships in the rat uterus.
Implantation, transplantation, and epithelial-mesenchymal relationships in the rat uterus.
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大鼠子宫中的植入，移植和上皮间质关系。
DOI：
10.1084/jem.132.4.721
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发表时间：
1970-10-01
影响因子：
15.3
通讯作者：
BILLINGHAM, RE
中科院分区：
文献类型：
--
作者：
BEER, AE;BILLINGHAM, RE
游离的尾部皮肤移植物或活表皮细胞的悬浮液已被放置在同源大鼠宿主的无损伤子宫中，并允许其自行“植入”，以研究该位点对于自然移植物（即受孕体）的可能独特性，及其对非自然移植物的反应。尽管存在完整的子宫内膜上皮，但只要在移植时建立雌激素过量状态，游离皮肤移植物就会迅速愈合。在缺乏雌激素的情况下，大多数移植物无法植入。一旦建立，移植物将无限期存活，无需进一步的雌激素。然而，如果在任何阶段建立持续发情状态，皮肤表皮会从移植物周边离心迁移，侵入性地取代天然子宫上皮。对子宫内雌激素促进表皮迁移的操作方式的分析结果支持了激素作用于子宫基质而不是表皮细胞的观点。当将舌粘膜或阴道“皮肤”的移植物放入处于长期发情状态的大鼠的子宫中时，表皮的迁移甚至比尾部皮肤更剧烈。当这些上皮细胞作为异型重组体在子宫的外来间充质基质上生长时，无限期地保留了其独特的组织学特异性。表皮细胞的单分散悬浮液似乎仅在子宫中预定接受受孕的部位“植入”并建立小表皮斑块。研究发现，在没有外源激素的情况下，两种移植物都可以在同一个子宫角中建立，这表明在子宫中建立皮肤移植物的内分泌参数与囊胚的内分泌参数相似。
Free tail skin grafts or suspensions of viable epidermal cells have been placed in the atraumatized uterus of isologous rat hosts and allowed to "implant" of their own accord to study the possible uniqueness of this site for other than nature's transplants, i.e. conceptuses, and its response to unnatural grafts. Despite the presence of an intact endometrial epithelium, free skin grafts heal-in rapidly, provided that a state of estrogen excess is established at the time of transplantation. In the absence of estrogen most of the grafts failed to implant. Once established, the grafts survive indefinitely without further estrogen. However, if at any stage a state of continual estrus is established, skin epidermis migrates centrifugally from the graft perimeter invasively replacing the native uterine epithelium. The results of an analysis of the modus operandi of this estrogen-facilitated epidermal migration in utero sustain the view that the hormone acts upon the uterine stroma rather than upon the epidermal cells. When grafts of lingual mucosa or vaginal "skin" were placed in the uteri of rats maintained in chronic estrus, migration of epidermis took place even more vigorously than from tail skin. These epithelia conserved their distinctive histologic specificities indefinitely when growing as heterotypic recombinants on the alien mesenchymal stroma of the uterus. Monodisperse suspensions of epidermal cells appear to "implant" and establish small epidermal plaques in the uterus only at sites predestined to accept conceptuses. That the endocrinologic parameters for the establishment of skin grafts in the uterus are similar to those for blastocysts is suggested by the finding that both kinds of graft can become established in the same uterine horn in the absence of exogenous hormones.
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DOI：
10.1016/s0007-1226(52)80004-9
发表时间：
1952-01-01
期刊：
BRITISH JOURNAL OF PLASTIC SURGERY
影响因子：
--
作者：
BILLINGHAM, RE;REYNOLDS, J
通讯作者：
REYNOLDS, J
影响因子：
7.1
作者：
BO, WJ
通讯作者：
BO, WJ
影响因子：
--
作者：
BO, WJ
通讯作者：
BO, WJ
影响因子：
64.8
作者：
MCLAREN, A
通讯作者：
MCLAREN, A
影响因子：
64.8
作者：
KIRBY, DRS
通讯作者：
KIRBY, DRS