Nanocomposite of Half-Fin Anchovy Hydrolysates/Zinc Oxide Nanoparticles Exhibits Actual Non-Toxicity and Regulates Intestinal Microbiota, Short-Chain Fatty Acids Production and Oxidative Status in Mice.
Nanocomposite of Half-Fin Anchovy Hydrolysates/Zinc Oxide Nanoparticles Exhibits Actual Non-Toxicity and Regulates Intestinal Microbiota, Short-Chain Fatty Acids Production and Oxidative Status in Mice.
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DOI：
10.3390/md16010023
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发表时间：
2018-01-11
期刊：
影响因子：
5.4
通讯作者：
Wei R
中科院分区：
文献类型：
--
作者：
Song R;Yao J;Shi Q;Wei R
根据我们先前的研究，与HAHp（3.0）或ZnO NPs相比，半鳍鱼水解物（HAHp）和氧化锌纳米颗粒（ZnO NPs）的纳米复合物（命名为HAHp（3.0）/ZnO NPs）表现出增加的抗菌活性。此外，在用HAHp（3.0）/ZnO NPs处理后，在大肠杆菌细胞中检测到活性氧（ROS）的形成。本研究的目的是评估这种纳米复合材料的急性毒性，并研究其对健康小鼠肠道微生物群组成，短链脂肪酸（SCFAs）生产和氧化状态的影响。限度试验研究表明，该纳米颗粒在试验剂量下无毒。HAHp（3.0）/ZnO纳米粒以1.0g/kg体重/d的剂量连续给药14天，空肠杯状细胞数量增加。粪便样品的高通量16 S核糖体RNA基因测序显示，HAHp（3.0）/ZnO NPs增加了雌性小鼠中的厚壁菌门（Firmicutes）并减少了拟杆菌门（Bacteriodetes）的丰度。此外，益生菌型细菌的微生物群，包括乳杆菌属和双歧杆菌属，以及梭菌属中的SCFA产生细菌，例如，未分类毛螺菌科和UCG-001毛螺菌科在雌性小鼠粪便中富集。SCFA的增加，特别是统计学上增加的丙酸和丁酸，表明HAHp（3.0）/ZnO纳米颗粒的上调抗炎活性。此外，肝脏中的一些阳性反应，如谷胱甘肽显著增加和丙二醛含量降低，表明氧化状态改善。因此，我们的研究结果表明，HAHp（3.0）/ZnO纳米颗粒可能具有潜在的应用，作为一个安全的肠道微生物群的调节剂，也可以用作食品中的抗氧化剂。
The nanocomposite of half-fin anchovy hydrolysates (HAHp) and zinc oxide nanoparticles (ZnO NPs) (named as HAHp(3.0)/ZnO NPs) demonstrated increased antibacterial activity compared to either HAHp(3.0) or ZnO NPs as per our previous studies. Also, reactive oxygen species (ROS) formation was detected in Escherichia coli cells after treatment with HAHp(3.0)/ZnO NPs. The aim of the present study was to evaluate the acute toxicity of this nanocomposite and to investigate its effect on intestinal microbiota composition, short-chain fatty acids (SCFAs) production, and oxidative status in healthy mice. The limit test studies show that this nanoparticle is non-toxic at the doses tested. The administration of HAHp(3.0)/ZnO NPs, daily dose of 1.0 g/kg body weight for 14 days, increased the number of goblet cells in jejunum. High-throughput 16S ribosomal RNA gene sequencing of fecal samples revealed that HAHp(3.0)/ZnO NPs increased Firmicutes and reduced Bacteriodetes abundances in female mice. Furthermore, the microbiota for probiotic-type bacteria, including Lactobacillus and Bifidobacterium, and SCFAs-producing bacteria in the Clostridia class, e.g., Lachnospiraceae_unclassified and Lachnospiraceae_UCG-001, were enriched in the feces of female mice. Increases of SCFAs, especially statistically increased propionic and butyric acids, indicated the up-regulated anti-inflammatory activity of HAHp(3.0)/ZnO NPs. Additionally, some positive responses in liver, like markedly increased glutathione and decreased malonaldehyde contents, indicated the improved oxidative status. Therefore, our results suggest that HAHp(3.0)/ZnO NPs could have potential applications as a safe regulator of intestinal microbiota or also can be used as an antioxidant used in food products.
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影响因子：
5.6
作者：
Arias, Maykel;Cobo, Marta;Del Campo, Rosa
通讯作者：
Del Campo, Rosa
影响因子：
8.1
作者：
Delgado-Andrade, Cristina;Pastoriza de la Cueva, Silvia;Rubio, Luis A.
通讯作者：
Rubio, Luis A.
影响因子：
5.4
作者：
Holzapfel, WH;Haberer, P;Huis in't Veld, JHJ
通讯作者：
Huis in't Veld, JHJ
在饮食中添加核桃后，肠道微生物群落的变化。
DOI：
10.1016/j.jnutbio.2017.07.001
发表时间：
2017-10
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Byerley LO;Samuelson D;Blanchard E 4th;Luo M;Lorenzen BN;Banks S;Ponder MA;Welsh DA;Taylor CM
通讯作者：
Taylor CM
影响因子：
3
作者：
Jayarambabu, N.;Rao, K. Venkateswara;Rajendar, V.
通讯作者：
Rajendar, V.