Changes in the gut microbial communities following addition of walnuts to the diet.
Changes in the gut microbial communities following addition of walnuts to the diet.
复制标题
在饮食中添加核桃后，肠道微生物群落的变化。
DOI：
10.1016/j.jnutbio.2017.07.001
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Taylor CM
中科院分区：
文献类型：
--
作者：
Byerley LO;Samuelson D;Blanchard E 4th;Luo M;Lorenzen BN;Banks S;Ponder MA;Welsh DA;Taylor CM
核桃富含omega-3脂肪酸、植物化学物质和抗氧化剂，与其他食物相比，核桃是独一无二的。食用核桃对健康有益，包括降低患心脏病和癌症的风险。肠道菌群失调与几种慢性疾病有关。核桃发挥其健康益处的一个潜在机制是通过改变肠道微生物群。这项研究确定了在饮食中加入核桃后肠道微生物群落的变化。雄性Fischer 344大鼠（n=20）被随机分配到两种饮食中的一种，为期10周：(1)核桃(W)和(2)替代(R)，其中核桃中的脂肪，纤维和蛋白质与玉米油，蛋白质酪蛋白和纤维素纤维来源相匹配。从降结肠收集肠道样本，分离DNA，并在Illumina MiSeq上对16S rRNA基因的V3-V4高变区进行深度测序，以表征肠道微生物群。两个饮食组的体重和食物摄入量没有显著差异。饮食组有不同的微生物群落，食用核桃的动物显示出更大的物种多样性。核桃增加了厚壁菌门的丰度，减少了细菌门的丰度。核桃丰富了益生菌型细菌的菌群，包括乳杆菌、瘤胃球菌科和玫瑰菌科，同时显著减少了拟杆菌和厌氧树干。α变形菌纲也减少了。食用核桃改变了肠道微生物群落，这表明核桃可能具有有益健康的作用的新机制。
Walnuts are rich in omega-3 fatty acids, phytochemicals and antioxidants making them unique compared to other foods. Consuming walnuts has been associated with health benefits including a reduced risk of heart disease and cancer. Dysbiosis of the gut microbiome has been linked to several chronic diseases. One potential mechanism by which walnuts may exert their health benefit is through modifying the gut microbiome. This study identified the changes in the gut microbial communities that occur following the inclusion of walnuts in the diet. Male Fischer 344 rats (n=20) were randomly assigned to one of two diets for as long as 10 weeks: (1) walnut (W), and (2) replacement (R) in which the fat, fiber, and protein in walnuts were matched with corn oil, protein casein, and a cellulose fiber source. Intestinal samples were collected from the descending colon, the DNA isolated, and the V3-V4 hypervariable region of 16S rRNA gene deep sequenced on an Illumina MiSeq for characterization of the gut microbiota. Body weight and food intake did not differ significantly between the two diet groups. The diet groups had distinct microbial communities with animals consuming walnuts displaying significantly greater species diversity. Walnuts increased the abundance of Firmicutes and reduced the abundance of Bacteriodetes. Walnuts enriched the microbiota for probiotic-type bacteria including Lactobacillus, Ruminococcaceae, and Roseburia while significantly reducing Bacteroides and Anaerotruncus. The class Alphaproteobacteria was also reduced. Walnut consumption altered the gut microbial community suggesting a new mechanism by which walnuts may confer their beneficial health effects.
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影响因子：
8.1
作者：
Lerin C;Goldfine AB;Boes T;Liu M;Kasif S;Dreyfuss JM;De Sousa-Coelho AL;Daher G;Manoli I;Sysol JR;Isganaitis E;Jessen N;Goodyear LJ;Beebe K;Gall W;Venditti CP;Patti ME
通讯作者：
Patti ME
影响因子：
4.7
作者：
Akinsete JA;Ion G;Witte TR;Hardman WE
通讯作者：
Hardman WE
影响因子：
7.1
作者：
Halvorsen, Bente L.;Carlsen, Monica H.;Blomhoff, Rune
通讯作者：
Blomhoff, Rune
影响因子：
10.6
作者：
Bruce-Keller AJ;Salbaum JM;Luo M;Blanchard E 4th;Taylor CM;Welsh DA;Berthoud HR
通讯作者：
Berthoud HR
DOI：
10.3945/jn.111.147660
发表时间：
2012-05
期刊：
The Journal of nutrition
影响因子：
--
作者：
Conlon MA;Kerr CA;McSweeney CS;Dunne RA;Shaw JM;Kang S;Bird AR;Morell MK;Lockett TJ;Molloy PL;Regina A;Toden S;Clarke JM;Topping DL
通讯作者：
Topping DL