Roles for RNA export factor, Nxt1, in ensuring muscle integrity and normal RNA expression in Drosophila.
Roles for RNA export factor, Nxt1, in ensuring muscle integrity and normal RNA expression in Drosophila.
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DOI：
10.1093/g3journal/jkaa046
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发表时间：
2021-01-18
期刊：
影响因子：
--
通讯作者：
White-Cooper H
中科院分区：
文献类型：
--
作者：
van der Graaf K;Jindrich K;Mitchell R;White-Cooper H
mRNA输出通路负责mRNA从细胞核转运到细胞质，因此对蛋白质产生和正常细胞功能至关重要。果蝇mRNA输出因子Nxt1功能等位基因的部分缺失表明生存能力和不育性降低。先前的一项研究表明，男性生育缺陷是由于转录和RNA稳定性的缺陷，这表明该途径可能涉及已知mRNA转运功能之外的过程。在这里，我们研究了Nxt1部分功能丧失突变体的生存能力降低，并描述了幼虫肌肉生长和维持的缺陷，导致肌肉变性。RNA-seq显示Nxt1突变体中一组mrna的表达减少，特别是来自长内含子的基因。我们检测到circRNA的差异表达，并且在突变体中表达的不同circRNA明显减少。尽管存在广泛的基因表达缺陷，但肌肉变性是通过增加肌肉中肋隔成分tn （abba）的表达来挽救的。这是首次报道RNA输出通路基因Nxt1在维持肌肉完整性中的作用。我们的数据还将mRNA输出途径与体内常见前体基因mRNA和circRNA表达中的特定作用联系起来。
The mRNA export pathway is responsible for the transport of mRNAs from the nucleus to the cytoplasm, and thus is essential for protein production and normal cellular functions. A partial loss of function allele of the mRNA export factor Nxt1 in Drosophila shows reduced viability and sterility. A previous study has shown that the male fertility defect is due to a defect in transcription and RNA stability, indicating the potential for this pathway to be implicated in processes beyond the known mRNA transport function. Here we investigate the reduced viability of Nxt1 partial loss of function mutants, and describe a defect in growth and maintenance of the larval muscles, leading to muscle degeneration. RNA-seq revealed reduced expression of a set of mRNAs, particularly from genes with long introns in Nxt1 mutant carcass. We detected differential expression of circRNA, and significantly fewer distinct circRNAs expressed in the mutants. Despite the widespread defects in gene expression, muscle degeneration was rescued by increased expression of the costamere component tn (abba) in muscles. This is the first report of a role for the RNA export pathway gene Nxt1 in the maintenance of muscle integrity. Our data also links the mRNA export pathway to a specific role in the expression of mRNA and circRNA from common precursor genes, in vivo.
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影响因子：
4
作者：
Kim JH;Jin P;Duan R;Chen EH
通讯作者：
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影响因子：
3
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4.5
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White-Cooper H
影响因子：
14.9
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影响因子：
14.9
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通讯作者：
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