Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: roles of oxidative and nitrosative stress and inducible nitric oxide synthase.
Adiponectin reduces carotid atherosclerotic plaque formation in ApoE-/- mice: roles of oxidative and nitrosative stress and inducible nitric oxide synthase.
复制标题
DOI：
10.3892/mmr.2014.2947
复制
发表时间：
2015-03
影响因子：
3.4
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Cai X;Li X;Li L;Huang XZ;Liu YS;Chen L;Zhang K;Wang L;Li X;Song J;Li S;Zhang Y;Zhang M
脂联素(APN)是一种重要的抗动脉粥样硬化脂肪细胞因子。本研究的目的是探讨脂联素在动脉粥样硬化斑块形成中的作用并阐明其机制。在雄性载脂蛋白E基因缺陷(ApoE−/−)小鼠左侧颈总动脉内放置致密性二氧化硅环，建立动脉粥样硬化模型。所有小鼠均喂饲高脂饲料，随机分为磷酸盐缓冲液处理组、腺病毒(Ad)-β-半乳糖苷酶处理组和Ad-APN处理组。与Ad-β-Gal或PBS治疗组相比，Ad-APN治疗组明显减少诱导型一氧化氮合酶(INOS)蛋白表达，减少一氧化氮/超氧化物的生成，阻断过氧亚硝酸盐的形成，逆转动脉粥样硬化病变的进展。脂联素可能是一种天然分子，通过抑制iNOS从而减轻氧化/硝化应激来减轻动脉粥样硬化。
Adiponectin (APN) is an important anti-atherogenic adipocytokine. The aim of the present study was to investigate the role of adiponectin in atherosclerotic plaque formation and clarify its mechanisms. An atherosclerosis model was induced by in vivo perivascular constrictive silica collar placement on the left common carotid arteries in male apolipoprotein E-deficient (ApoE−/−) mice. All of the mice were fed a high-fat diet, and divided into phosphate-buffered saline, adenovirus (Ad)-β-galactosidase and Ad-APN treatment groups. Compared with treatment of Ad-β-gal or PBS, Ad-APN treatment markedly reduced inducible nitric oxide synthase (iNOS) protein expression, decreased in nitric oxide/superoxide production, blocked peroxynitrite formation and reversed the progression of atherosclerotic lesions. Adiponectin may be a natural molecule that reduces atherosclerosis by inhibiting iNOS and consequently diminishing oxidative/nitrative stress.
登录
查看更多内容
影响因子：
9.3
作者：
Sulistyoningrum DC;Gasevic D;Lear SA;Ho J;Mente A;Devlin AM
通讯作者：
Devlin AM
影响因子：
37.8
作者：
Okamoto, Y;Kihara, S;Matsuzawa, Y
通讯作者：
Matsuzawa, Y
影响因子：
8.7
作者：
Hotta, K;Funahashi, T;Matsuzawa, Y
通讯作者：
Matsuzawa, Y
影响因子：
37.8
作者：
Tao, L;Liu, HR;Yue, TL
通讯作者：
Yue, TL
影响因子：
7.4
作者：
Gozin, A;Franzini, E;Pasquier, C
通讯作者：
Pasquier, C