Microfabricated polymeric vessel mimetics for 3-D cancer cell culture.
Microfabricated polymeric vessel mimetics for 3-D cancer cell culture.
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DOI：
10.1016/j.biomaterials.2013.07.013
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发表时间：
2013-11
期刊：
影响因子：
14
通讯作者：
Gottesman, Michael M.
中科院分区：
文献类型：
--
作者：
Jaeger, Ashley A.;Das, Chandan K.;Morgan, Nicole Y.;Pursley, Randall H.;McQueen, Philip G.;Hall, Matthew D.;Pohida, Thomas J.;Gottesman, Michael M.
体外肿瘤生长模型对于临床前癌症研究中假设的成本效益测试至关重要。3-D细胞培养提供了一个改进的单层培养研究癌症生物学中的细胞过程，因为保存细胞-细胞和细胞-ECM的相互作用。氧转运对模拟体内环境构成主要障碍，并且在常规细胞培养系统中不能复制。我们假设，我们可以使用生物反应器系统更好地模拟肿瘤微环境，用于控制具有硅水凝胶合成血管的癌细胞培养物中的气体交换。软光刻技术被用来制造氧渗透性硅水凝胶膜含有阵列的微柱。将这些膜插入生物反应器中，并由基底膜提取物（BME）包围，在基底膜提取物（BME）中培养荧光卵巢癌（OVCAR 8）细胞。通过合成血管充氧的细胞簇显示出100 μ m的缺氧下降，这与微血管系统喂养的肿瘤结节的体内研究一致。我们发现，通过合成血管充氧的簇内的氧张力梯度在缺氧时下降了100 µm，这与体内研究一致。在生物反应器系统中的氧传输的特点是通过实验测试与溶解氧探头和对流的有限元建模。我们的研究证明了与控制气体分布以更好地模拟体内条件相关的不同生长模式。
Modeling tumor growth in vitro is essential for cost-effective testing of hypotheses in preclinical cancer research. 3-D cell culture offers an improvement over monolayer culture for studying cellular processes in cancer biology because of the preservation of cell-cell and cell-ECM interactions. Oxygen transport poses a major barrier to mimicking in vivo environments and is not replicated in conventional cell culture systems. We hypothesized that we can better mimic the tumor microenvironment using a bioreactor system for controlling gas exchange in cancer cell cultures with silicone hydrogel synthetic vessels. Soft-lithography techniques were used to fabricate oxygen-permeable silicone hydrogel membranes containing arrays of micropillars. These membranes were inserted into a bioreactor and surrounded by basement membrane extract (BME) within which fluorescent ovarian cancer (OVCAR8) cells were cultured. Cell clusters oxygenated by synthetic vessels showed a ∼100um drop-off to anoxia, consistent with in vivo studies of tumor nodules fed by the microvasculature. We showed oxygen tension gradients inside the clusters oxygenated by synthetic vessels had a ∼100 µm drop-off to anoxia, which is consistent with in vivo studies. Oxygen transport in the bioreactor system was characterized by experimental testing with a dissolved oxygen probe and finite element modeling of convective flow. Our study demonstrates differing growth patterns associated with controlling gas distributions to better mimic in vivo conditions.
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影响因子：
3
作者：
Gerlach, Joerg C.;Lin, Yen-Chih;Marra, Kacey G.
通讯作者：
Marra, Kacey G.
影响因子：
50.3
作者：
Hanahan, Douglas;Coussens, Lisa M.
通讯作者：
Coussens, Lisa M.
影响因子：
14
作者：
Curcio, Efrern;Salerno, Simona;Bader, Augustinus
通讯作者：
Bader, Augustinus
DOI：
10.2217/nnm.10.12
发表时间：
2010-04
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Geckil H;Xu F;Zhang X;Moon S;Demirci U
通讯作者：
Demirci U
影响因子：
6.1
作者：
Ainslie, Kristy M.;Kraning, Casey M.;Desai, Tejal A.
通讯作者：
Desai, Tejal A.