Characterization of murine cytomegalovirus infection and induction of calcification in Murine Aortic Vascular Smooth Muscle Cells (MOVAS).
Characterization of murine cytomegalovirus infection and induction of calcification in Murine Aortic Vascular Smooth Muscle Cells (MOVAS).
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DOI：
10.1016/j.jviromet.2021.114270
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发表时间：
2021-11
影响因子：
3.1
通讯作者：
Cardin RD
中科院分区：
文献类型：
--
作者：
Bonavita CM;White TM;Stanfield BA;Cardin RD
人巨细胞病毒（HCMV）是一种广泛分布的病原体，在世界大多数人口中引起终身潜伏感染。HCMV与许多心血管疾病的发病率和严重程度增加相关，包括心肌炎、动脉粥样硬化和移植血管病。由于巨细胞病毒感染的物种限制性，鼠巨细胞病毒（MCMV）是一个有用的模型，概括了心血管系统HCMV感染的许多特征。虽然体内MCMV研究能够回答有关感染发病机制的许多问题，但使用细胞系的体外实验是进一步了解潜在潜在机制的有用工具。在这项研究中，我们的特点MCMV感染的小鼠主动脉平滑肌细胞系（MOVAS）。我们的研究结果表明，MOVAS细胞允许MCMV感染，在羧甲基纤维素覆盖下形成噬斑，并产生与NIH 3T3小鼠胚胎成纤维细胞相似的子代病毒。此外，MCMV感染诱导MOVAS细胞钙化，类似于MCMV感染心脏的心外膜。我们的结论是，MOVAS细胞是一个有用的体外研究CMV介导的心脏钙化的工具。
Human cytomegalovirus (HCMV) is a widespread pathogen that causes lifelong latent infection in the majority of the world population. HCMV is associated with increased incidence and severity of many cardiovascular diseases including myocarditis, atherosclerosis, and transplant vasculopathy. Due to the species-restricted nature of cytomegalovirus infection, murine cytomegalovirus (MCMV) is a useful model that recapitulates many of the features of HCMV infection of the cardiovascular system. While in vivo MCMV studies are able to answer many questions regarding pathogenesis of infection, in vitro experiments using cell lines are useful tools to further understand the potential underlying mechanisms. In this study, we characterize MCMV infection of the murine aortic smooth muscle cell line (MOVAS). Our findings demonstrate that MOVAS cells are permissive for MCMV infection, form plaques under carboxymethyl cellulose overlay, and produce progeny virus similar to NIH 3T3 murine embryonic fibroblasts. In addition, MCMV infection induces calcification in MOVAS cells similar to that seen in the epicardium of MCMV-infected hearts. We conclude that MOVAS cells are a useful in vitro tool for studying CMV-mediated cardiac calcification.
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