Effect of tonsillar mononuclear cell supernatants in patients with IgA nephropathy on renal tubular epithelial cells
Effect of tonsillar mononuclear cell supernatants in patients with IgA nephropathy on renal tubular epithelial cells
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IgA肾病患者扁桃体单个核细胞上清液对肾小管上皮细胞的影响
DOI：
10.1007/s00011-012-0549-0
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发表时间：
2012
影响因子：
6.7
通讯作者：
Liu, Fuyou
中科院分区：
文献类型：
--
作者：
Yang, Sha;Liu, Hong;Wu, Gang;Liu, Fuyou
本研究旨在探讨扁桃体炎症与IgA肾病（IgAN）的免疫学关系及发病机制。方法采用植物血凝素（PHA）诱导人肾小管上皮细胞（HK-2）炎症反应，观察肾小管上皮细胞分泌物对肾小管上皮细胞增殖的影响。收集经PHA刺激或不经PHA刺激的TMCs分泌物，并将其应用于HK-2细胞。观察HK-2细胞增殖、凋亡及上皮间质转化（EMT）情况。结果PHA刺激的IgANTMCs分泌物对HK-2细胞的增殖有明显的抑制作用，对细胞凋亡有明显的促进作用，对Bcl-2的表达有明显的下调作用（P< 0.05），并呈时间和浓度依赖性。结论PHA刺激的IgANTMCs分泌物可抑制HK-2 TEC的增殖，促进其凋亡，下调Bcl-2的表达，并具有EMT效应，可能反映了扁桃体炎症对肾TEC功能的体内远程调节。
ObjectiveThis study aimed to investigate the immunological relationship and the mechanism between tonsillar inflammation and immunoglobulin A nephropathy (IgAN).SubjectsTonsillar mononuclear cells (TMCs) were prepared from 13 patients with IgAN and 13 patients with chronic tonsillitis but without renal disease. The human renal tubular epithelial cell (TEC) line, HK-2, was used to test the effects of secretions from the TMCs.MethodsPhytohemagglutinin (PHA) was used to induce the inflammatory responses in TMCs. The secretions from TMCs, stimulated with or without PHA, were collected and applied to HK-2 cells. The proliferation, apoptosis, and epithelial–mesenchymal transition (EMT) of HK-2 cells were evaluated. The expression of key components during apoptosis and EMT was measured.ResultsThe secretions from PHA-stimulated IgAN TMCs significantly inhibited proliferation, promoted apoptosis, and down-regulated Bcl-2 in HK-2 cells (P< 0.05) in time- and concentration-dependent manners. They also modulated the expression of key components during EMT, E-cadherin and α-SMA (P< 0.05).ConclusionsThe secretions from PHA-stimulated IgAN TMCs can cause the inhibition of proliferation, promotion of apoptosis, down-regulation of Bcl-2, and EMT effects in HK-2 TECs, which may reflect the in-vivo remote modulation of functions of renal TECs by tonsillar inflammation.
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影响因子：
--
作者：
John Calvin Reed;T. Miyashita;S. Krajewski;S. Takayama;C. Aimé-sempé;S. Kitada;T. Sato;H. G. Wang;M. Harigai;M. Hanada;M. Krajewska;K. Kochel;J. Millán;H. Kobayashi
通讯作者：
John Calvin Reed;T. Miyashita;S. Krajewski;S. Takayama;C. Aimé-sempé;S. Kitada;T. Sato;H. G. Wang;M. Harigai;M. Hanada;M. Krajewska;K. Kochel;J. Millán;H. Kobayashi
DOI：
10.1084/jem.157.2.572
发表时间：
1983-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Emancipator SN;Gallo GR;Lamm ME
通讯作者：
Lamm ME
DOI：
10.1016/s0021-9258(18)38177-8
发表时间：
1990-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Perumal Thiagarajan;Jonathan F. Tait
通讯作者：
Perumal Thiagarajan;Jonathan F. Tait
DOI：
10.1056/nejm198111053051922
发表时间：
1981-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Y. Tomino;M. Endoh;Y. Nomoto;H. Sakai
通讯作者：
Y. Tomino;M. Endoh;Y. Nomoto;H. Sakai
影响因子：
4.5
作者：
Huh J;Chi HS;Kim SS;Gong G
通讯作者：
Gong G