Enhancing the precision of genetic lineage tracing using dual recombinases.
Enhancing the precision of genetic lineage tracing using dual recombinases.
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使用双重组酶提高遗传谱系追踪的精度。
DOI：
10.1038/nm.4437
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发表时间：
2017-12
期刊：
影响因子：
82.9
通讯作者：
Zhou B
中科院分区：
文献类型：
--
作者：
He L;Li Y;Li Y;Pu W;Huang X;Tian X;Wang Y;Zhang H;Liu Q;Zhang L;Zhao H;Tang J;Ji H;Cai D;Han Z;Han Z;Nie Y;Hu S;Wang QD;Sun R;Fei J;Wang F;Chen T;Yan Y;Huang H;Pu WT;Zhou B
Cre-loxP重组系统是用于体内追踪干细胞或祖细胞谱系的最广泛的技术。这种遗传系统的精确度在很大程度上取决于Cre在靶向干细胞或祖细胞中表达的特异性。然而，Cre在非靶向细胞类型中的表达可能会使谱系追踪研究的解释复杂化，并在许多先前的研究中引起争议。在这里，我们描述了一种新的遗传血统追踪系统，它结合了DRE-ROX来提高传统的Cre-loxP介导的血统追踪的精确度。Dre-Rox重组系统允许对潜在的非故意Cre-loxP重组进行严格控制，有效地避免了谱系追踪中Cre表达的细胞类型特异性的不确定性。利用这一新系统，我们研究了最近争论的两个话题：c-Kit+心脏干细胞对心脏心肌细胞的贡献和Sox9+肝祖细胞对肝脏肝细胞的贡献。通过克服非特异性Cre表达的技术障碍，这项新技术为更精确地分析细胞谱系和命运决定提供了一种手段，促进了在体内研究疾病和再生中干细胞和祖细胞的可塑性。小鼠的遗传细胞谱系追踪研究对于描绘干细胞和祖细胞对不同细胞类型的贡献至关重要，无论是在疾病状态下还是在再生治疗后。他等人。已经开发了新的遗传谱系追踪系统，提供了比常用的基于CRE的系统更明确的结果，并表明这一新技术可以解决该领域目前的争议，心脏和肝脏的谱系追踪研究证明了这一点。
The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre expression in targeted stem or progenitor cells. However, Cre expression in non-targeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage-tracing system that incorporates Dre-rox to enhance the precision of conventional Cre-loxP mediated lineage tracing. The Dre-rox recombination system permits rigorous control of potential unintentional Cre-loxP recombination, effectively circumventing the uncertainty of the cell-type specificity of Cre expression in lineage tracing. Using this new system, we investigated two topics of recent debates: the contribution of c-Kit+ cardiac stem cells to cardiomyocytes in the heart and the contribution of Sox9+ hepatic progenitor cells to hepatocytes in the liver. By overcoming the technical hurdle of non-specific Cre expression, this new technology provides a means for more precise analysis of cell lineage and fate decisions, facilitating in vivo study of stem and progenitor cell plasticity in disease and regeneration. Genetic cell-lineage tracing studies in mice are crucial for delineating the contribution of stem and progenitor cells to different cell types, both in disease states and after regenerative therapy. He et al. have developed new genetic lineage tracing systems that provide more definitive results than the commonly used Cre-based system and show that this new technology can resolve current controversies in the field, as demonstrated by lineage tracing studies in the heart and liver.
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影响因子：
20.1
作者：
Hatzistergos KE;Saur D;Seidler B;Balkan W;Breton M;Valasaki K;Takeuchi LM;Landin AM;Khan A;Hare JM
通讯作者：
Hare JM
影响因子：
64.8
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
影响因子：
1.5
作者：
Guo, CY;Yang, WY;Lobe, CG
通讯作者：
Lobe, CG
影响因子：
64.5
作者：
Ellison, Georgina M.;Vicinanza, Carla;Nadal-Ginard, Bernardo
通讯作者：
Nadal-Ginard, Bernardo
影响因子：
82.9
作者：
Liu, Qiaozhen;Huang, Xiuzhen;Zhou, Bin
通讯作者：
Zhou, Bin