Major histocompatibility complex based resistance to a common bacterial pathogen of amphibians.
Major histocompatibility complex based resistance to a common bacterial pathogen of amphibians.
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DOI：
10.1371/journal.pone.0002692
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发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Waldman, Bruce
中科院分区：
文献类型：
--
作者：
Barribeau, Seth M.;Villinger, Jandouwe;Waldman, Bruce
考虑到它们发达的先天免疫和适应性免疫系统，全球两栖动物数量的下降尤其令人困惑。为了研究主要组织相容性复合体（MHC）在赋予病原体抗性中的作用，我们在两个实验中对携带4种MHC单倍型（f、g、j和r）不同组合的非洲爪蟾蝌蚪进行了嗜水气单胞菌感染。首先，我们将从MHC纯合亲本中获得的ff， fg, gg， gj和jj蝌蚪暴露于三种剂量的嗜水单胞杆菌或热杀细菌中的一种作为对照。在第二项研究中，我们将从MHC杂合亲本中获得的ff、fg、fr、gg、rg和rr蝌蚪暴露于嗜水单胞杆菌、热杀细菌或单独培养基中作为对照，然后通过PCR进行基因分型。因此，我们确定了MHC耐药模式是否与家族之间相同，独立于非MHC遗传差异。拥有r或g个MHC单倍型的蝌蚪比拥有j个MHC单倍型的蝌蚪更容易死亡。不同类型MHC的生长速率不同，与暴露剂量无关。具有易感单倍型和抗性单倍型的杂合子个体在大小和存活率上均处于纯合子基因型的中间。MHC对生长和生存的影响在不同实验和不同家庭之间是一致的。MHC等位基因对影响蝌蚪生长和存活的细菌病原体具有不同的抵抗力或耐受性。
Given their well-developed systems of innate and adaptive immunity, global population declines of amphibians are particularly perplexing. To investigate the role of the major histocompatibilty complex (MHC) in conferring pathogen resistance, we challenged Xenopus laevis tadpoles bearing different combinations of four MHC haplotypes (f, g, j, and r) with the bacterial pathogen Aeromonas hydrophila in two experiments. In the first, we exposed ff, fg, gg, gj, and jj tadpoles, obtained from breeding MHC homozygous parents, to one of three doses of A. hydrophila or heat-killed bacteria as a control. In the second, we exposed ff, fg, fr, gg, rg, and rr tadpoles, obtained from breeding MHC heterozygous parents and subsequently genotyped by PCR, to A. hydrophila, heat-killed bacteria or media alone as controls. We thereby determined whether the same patterns of MHC resistance emerged within as among families, independent of non-MHC heritable differences. Tadpoles with r or g MHC haplotypes were more likely to die than were those with f or j haplotypes. Growth rates varied among MHC types, independent of exposure dose. Heterozygous individuals with both susceptible and resistant haplotypes were intermediate to either homozygous genotype in both size and survival. The effect of the MHC on growth and survival was consistent between experiments and across families. MHC alleles differentially confer resistance to, or tolerance of, the bacterial pathogen, which affects tadpoles' growth and survival.
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DOI：
10.1111/j.1749-6632.2002.tb04400.x
发表时间：
2002-01-01
期刊：
DOMESTIC ANIMAL/WILDLIFE INTERFACE: ISSUE FOR DISEASE CONTROL, CONSERVATION, SUSTAINABLE FOOD PRODUCTION, AND EMERGING DISEASES
影响因子：
--
作者：
Green, DE;Converse, KA;Schrader, AK
通讯作者：
Schrader, AK
影响因子：
56.9
作者：
Carrington, M;Nelson, GW;O'Brien, SJ
通讯作者：
O'Brien, SJ
影响因子：
0.8
作者：
BRADFORD, DF
通讯作者：
BRADFORD, DF
影响因子：
4.9
作者：
Acevedo-Whitehouse, K;Vicente, J;Amos, W
通讯作者：
Amos, W
影响因子：
2.6
作者：
CRUMP, ML
通讯作者：
CRUMP, ML