The transcriptionally active regions in the genome of Bacillus subtilis.
The transcriptionally active regions in the genome of Bacillus subtilis.
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DOI:
10.1111/j.1365-2958.2009.06830.x
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发表时间:
2009-09
影响因子:
3.6
通讯作者:
Jarmer H
中科院分区:
文献类型:
--
作者:
Rasmussen S;Nielsen HB;Jarmer H
The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3′ untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.
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影响因子:
4.3
作者:
de Hoon, Michiel J L;Makita, Yuko;Nakai, Kenta;Miyano, Satoru
通讯作者:
Miyano, Satoru
影响因子:
3.2
作者:
Gaidenko, Tatiana A.;Kim, Tae-Jong;Price, Chester W.
通讯作者:
Price, Chester W.
影响因子:
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作者:
Bertone, P;Stolc, V;Snyder, M
通讯作者:
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影响因子:
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作者:
Eiamphungporn, Warawan;Helmann, John D.
通讯作者:
Helmann, John D.
DOI:
10.1073/pnas.0711752105
发表时间:
2008-08-19
影响因子:
11.1
作者:
Gaballa, Ahmed;Antelmann, Haike;Helmann, John D.
通讯作者:
Helmann, John D.