The transcriptionally active regions in the genome of Bacillus subtilis.

The transcriptionally active regions in the genome of Bacillus subtilis.
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DOI:
10.1111/j.1365-2958.2009.06830.x
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发表时间:
2009-09
影响因子:
3.6
通讯作者:
Jarmer H
Jarmer H
中科院分区:
生物学2区
文献类型:
--
作者:
Rasmussen S;Nielsen HB;Jarmer H

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到目前为止,所有基因中的大多数已经通过计算机基因发现系统地鉴定和注释。在这里,我们报告的发现3662链特异性转录活性区(TAR)的枯草芽孢杆菌基因组中使用平铺阵列。我们已经测量了在富(LB)和最小(M9)培养基上的中期指数生长期间的全基因组表达。所确定的TAR占目前注释的基因的77.3%,此外,我们发现84个推定的非编码RNA(ncRNA)和127个反义转录本。一个ncRNA,ncr22,预计作为一个翻译控制cstA和反义转录物观察到相反的管家σ因子sigA。通过这项工作,我们在一组膜相关基因中发现了一个长时间保守的3′非翻译区(UTR),预计它将折叠成一个大而高度稳定的二级结构。具有该尾部的基因之一是efeN,其编码双精氨酸易位酶(达特)蛋白易位系统的靶标。
The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3′ untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.
枯草芽孢杆菌和相关物种中转录终止剂的预测。
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