White Collar 1 Modulates Oxidative Sensitivity and Virulence by Regulating the HOG1 Pathway in Fusarium asiaticum.
White Collar 1 Modulates Oxidative Sensitivity and Virulence by Regulating the HOG1 Pathway in Fusarium asiaticum.
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White Collar 1 通过调节 Fusarium asiaticum 中的 HOG1 途径来调节氧化敏感性和毒力。
DOI：
10.1128/spectrum.05206-22
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发表时间：
2023-06-15
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
亚洲镰刀菌（Fusarium asiaticum）是东亚地区重要的禾谷类作物病原菌，是造成粮食和饲料产量损失和真菌毒素污染的主要原因。FaWC 1是蓝光受体白色项圈复合物（WCC）的一个组成部分，其致病性的调控机制不是光氧电压结构域，而是转录调控锌指结构域。asiaticum，尽管下游机制仍然不清楚。本研究对FaWC 1调控的致病因子进行了分析。结果发现，FaWC 1的缺失导致对活性氧（ROS）的敏感性高于野生型，而外源性ROS淬灭剂抗坏血酸的应用使Δ Fawc 1菌株的致病性恢复到野生型的水平，表明Δ Fawc 1菌株的致病性降低是由于ROS耐受性缺陷。此外，高渗甘油（HOG）丝裂原活化蛋白激酶（MAPK）途径基因及其下游编码ROS清除酶的基因在Δ Fawc 1突变体中表达水平下调。在ROS刺激下，由天然启动子驱动的FaHOG 1-绿色荧光蛋白（GFP）表达信号在野生型中是可诱导的，但在Δ Fawc 1菌株中可忽略不计。在Δ Fawc 1中过量表达Fahog 1可以恢复Δ Fawc 1突变株的ROS耐受性和致病性，但仍存在光反应缺陷。综上所述，本研究分析了蓝光受体组分FaWC 1在调控细胞内HOG-MAPK信号通路表达水平从而影响F. p.亚洲人。重要性众所周知，保守的真菌蓝光受体白色项圈复合物（WCC）调节植物或人类宿主的几种致病性物种的毒力，但WCC如何决定真菌致病性仍然很大程度上未知。WCC组分FaWC 1在谷物病原体亚洲镰刀菌先前被发现是所需的全毒力。本研究探讨了FaWC 1在调控细胞内HOG MAPK信号通路中的作用，进而影响了F.t.亚洲人。因此，这项工作扩展了真菌光受体和细胞内应激信号通路之间的关联，以调节氧化胁迫耐受性和致病性的流行病学上重要的真菌病原体的谷类作物的知识。
Fusarium asiaticum is an epidemiologically important pathogen of cereal crops in east Asia, accounting for both yield losses and mycotoxin contamination problems in food and feed products. FaWC1, a component of the blue-light receptor White Collar complex (WCC), relies on its transcriptional regulatory zinc finger domain rather than the light-oxygen-voltage domain to regulate pathogenicity of F. asiaticum, although the downstream mechanisms remain obscure. In this study, the pathogenicity factors regulated by FaWC1 were analyzed. It was found that loss of FaWC1 resulted in higher sensitivity to reactive oxygen species (ROS) than in the wild type, while exogenous application of the ROS quencher ascorbic acid restored the pathogenicity of the ΔFawc1 strain to the level of the wild type, indicating that the reduced pathogenicity of the ΔFawc1 strain is due to a defect in ROS tolerance. Moreover, the expression levels of the high-osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK) pathway genes and their downstream genes encoding ROS scavenging enzymes were downregulated in the ΔFawc1 mutant. Upon ROS stimulation, the FaHOG1-green fluorescent protein (GFP)-expressing signal driven by the native promoter was inducible in the wild type but negligible in the ΔFawc1 strain. Overexpressing Fahog1 in the ΔFawc1 strain could recover the ROS tolerance and pathogenicity of the ΔFawc1 mutant, but it remained defective in light responsiveness. In summary, this study dissected the roles of the blue-light receptor component FaWC1 in regulating expression levels of the intracellular HOG-MAPK signaling pathway to affect ROS sensitivity and pathogenicity in F. asiaticum. IMPORTANCE The well-conserved fungal blue-light receptor White Collar complex (WCC) is known to regulate virulence of several pathogenic species for either plant or human hosts, but how WCC determines fungal pathogenicity remains largely unknown. The WCC component FaWC1 in the cereal pathogen Fusarium asiaticum was previously found to be required for full virulence. The present study dissected the roles of FaWC1 in regulating the intracellular HOG MAPK signaling pathway to affect ROS sensitivity and pathogenicity in F. asiaticum. This work thus extends knowledge of the association between fungal light receptors and the intracellular stress signaling pathway to regulate oxidative stress tolerance and pathogenicity in an epidemiologically important fungal pathogen of cereal crops.
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影响因子：
11.4
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
通讯作者：
Loros, Jennifer J.
影响因子：
56.9
作者：
Crosthwaite, SK;Dunlap, JC;Loros, JJ
通讯作者：
Loros, JJ
影响因子：
5.4
作者：
Molin M;Logg K;Bodvard K;Peeters K;Forsmark A;Roger F;Jörhov A;Mishra N;Billod JM;Amir S;Andersson M;Eriksson LA;Warringer J;Käll M;Blomberg A
通讯作者：
Blomberg A
影响因子：
--
作者：
Dexter JP;Xu P;Gunawardena J;McClean MN
通讯作者：
McClean MN
DOI：
10.1016/j.fgb.2018.09.008
发表时间：
2018-12
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Zhu P;Idnurm A
通讯作者：
Idnurm A