Low-Intensity Focused Ultrasound Alleviates Spasticity and Increases Expression of the Neuronal K-Cl Cotransporter in the L4-L5 Sections of Rats Following Spinal Cord Injury.
Low-Intensity Focused Ultrasound Alleviates Spasticity and Increases Expression of the Neuronal K-Cl Cotransporter in the L4-L5 Sections of Rats Following Spinal Cord Injury.
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DOI：
10.3389/fncel.2022.882127
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发表时间：
2022
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
作者：
低强度聚焦超声（LIFU）已被证明可以有效激活脊髓神经回路。本研究的目的是探讨LIFU的影响，以减轻痉挛脊髓损伤（SCI）后激活脊髓神经回路和增加神经元钾-氯转运蛋白KCC 2的表达。将成年雄性Sprague道利（SD）大鼠（220-300 g）随机分为假手术对照组、LIFU-组和LIFU+组。采用机械阈值保持（g）评价痉挛的行为学特征。肌电图（EMG）用于评估脊髓神经回路和肌肉自发收缩的激活。用频率依赖性抑郁（FDD）评估痉挛。采用免疫印迹法（WB）和免疫荧光法（IF）检测腰髓KCC 2的表达。通过LIFU模拟激活脊髓神经回路，其在治疗4周后显著降低机械阈值（g）、FDD和EMG记录。WB和IF染色均显示LIFU−组KCC 2表达减少（P < 0.05）。LIFU刺激4周后，LIFU+组与LIFU−组相比，KCC 2的表达显著增加（P < 0.05）。因此，我们假设LIFU治疗可以有效地缓解脊髓损伤大鼠L4-L5节段的痉挛状态，并上调KCC 2的表达。
Low-intensity focused ultrasound (LIFU) has been shown to provide effective activation of the spinal cord neurocircuits. The aim of this study was to investigate the effects of LIFU in order to alleviate spasticity following spinal cord injury (SCI) by activating the spinal neurocircuits and increasing the expression of the neuronal K-Cl cotransporter KCC2. Adult male Sprague Dawley (SD) rats (220–300 g) were randomly divided into a sham control group, a LIFU− group, and a LIFU+ group. The mechanical threshold hold (g) was used to evaluate the behavioral characteristics of spasm. Electromyography (EMG) was used to assess activation of the spinal cord neurocircuits and muscle spontaneous contraction. Spasticity was assessed by frequency-dependent depression (FDD). The expression of KCC2 of the lumbar spinal cord was determined via western blot (WB) and immunofluorescence (IF) staining. The spinal cord neurocircuits were activated by LIFU simulation, which significantly reduced the mechanical threshold (g), FDD, and EMG recordings (s) after 4 weeks of treatment. WB and IF staining both demonstrated that the expression of KCC2 was reduced in the LIFU− group (P < 0.05). After 4 weeks of LIFU stimulation, expression of KCC2 had significantly increased (P < 0.05) in the LIFU+ group compared with the LIFU− group. Thus, we hypothesized that LIFU treatment can alleviate spasticity effectively and upregulate the expression of KCC2 in the L4–L5 section of SCI rats.
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影响因子：
5.3
作者：
Cote, Marie-Pascale;Gandhi, Sapan;Houle, John D.
通讯作者：
Houle, John D.
DOI：
10.1016/j.apmr.2016.09.124
发表时间：
2017-06-01
影响因子：
4.3
作者：
Holtz, Kaila A.;Lipson, Rachel;Mills, Patricia B.
通讯作者：
Mills, Patricia B.
影响因子：
2.4
作者：
Ai L;Bansal P;Mueller JK;Legon W
通讯作者：
Legon W
影响因子：
3.7
作者：
Corleto JA;Bravo-Hernández M;Kamizato K;Kakinohana O;Santucci C;Navarro MR;Platoshyn O;Cizkova D;Lukacova N;Taylor J;Marsala M
通讯作者：
Marsala M
影响因子：
4.2
作者：
Beverungen, Henrike;Klaszky, Samantha Choyke;Cote, Marie-Pascale
通讯作者：
Cote, Marie-Pascale