The development of melanopsin-containing retinal ganglion cells in mice with early retinal degeneration.
The development of melanopsin-containing retinal ganglion cells in mice with early retinal degeneration.
复制标题
DOI：
10.1111/j.1460-9568.2008.06589.x
复制
发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Robinson DW
中科院分区：
文献类型：
--
作者：
Ruggiero L;Allen CN;Brown RL;Robinson DW
在哺乳动物中，视杆细胞和视锥细胞光感受器介导视觉的神经元通路已得到充分记录。然而，经典光感受器在光夹带中所起的作用尚不清楚。在哺乳动物中，表达感光色素黑视蛋白的本质光敏视网膜神经节细胞（ipRGC）直接投射到下丘脑的视交叉上核（SCN），即生物钟的位置，从而有助于对光的非图像形成反应。经典的光感受器对于光夹带来说不是必需的，因为视杆细胞和视锥细胞的损失并不能消除光夹带。然而，当在视网膜退化 CBA/J 小鼠中观察到减弱的相移反应时，出现了相互矛盾的证据。在这项研究中，我们检查了 CBA/J 小鼠视网膜变性的时间进程，并利用这些动物来确定外视网膜的成熟是否调节 ipRGC 的形态、数量和分布。我们还研究了外视网膜早期发育过程中的退化是否会改变成人昼夜节律系统的功能。我们报告说，在早期视网膜变性的小鼠中，ipRGC 的树突分层和分布没有改变，这表明外视网膜的正常发育对于这些过程不是必需的。然而，我们发现，成年 CBA/J 小鼠的 ipRGC 数量比对照小鼠多，这表明外部视网膜光感受器在调节 ipRGC 发育细胞死亡中发挥着作用。
In mammals, the neuronal pathways by which rod and cone photoreceptors mediate vision have been well documented. The roles that classical photoreceptors play in photoentrainment, however, have been less clear. In mammals, intrinsically photosensitive retinal ganglion cells (ipRGCs) that express the photopigment melanopsin project directly to the suprachiasmatic nucleus (SCN) of the hypothalamus, the site of the circadian clock, and thereby contribute to non-image forming responses to light. Classical photoreceptors are not necessary for photoentrainment since loss of rods and cones does not eliminate light entrainment. Conflicting evidence arose, however, when attenuated phase-shifting responses were observed in the retinal degenerate CBA/J mouse. In this study, we examined the time course of retinal degeneration in CBA/J mice and used these animals to determine if maturation of the outer retina regulates the morphology, number and distribution of ipRGCs. We also examined whether degeneration during the early development of the outer retina can alter the function of the adult circadian system. We report that dendritic stratification and distribution of ipRGCs was unaltered in mice with early retinal degeneration, suggesting that normal development of the outer retina was not necessary for these processes. We found, however, that adult CBA/J mice have greater numbers of ipRGCs than controls, implicating a role for the outer retinal photoreceptors in regulating developmental cell death of ipRGCs.
登录
查看更多内容
影响因子：
25
作者：
Gooley, JJ;Lu, J;Saper, CB
通讯作者：
Saper, CB
影响因子：
3.4
作者：
Perez-Leon, Jorge Alberto;Warren, Erin J.;Brown, R. Lane
通讯作者：
Brown, R. Lane
影响因子：
3.4
作者：
Warren, EJ;Allen, CN;Robinson, DW
通讯作者：
Robinson, DW
影响因子：
64.8
作者：
BODNARENKO, SR;CHALUPA, LM
通讯作者：
CHALUPA, LM
影响因子：
64.8
作者：
Gueler, Ali D.;Ecker, Jennifer L.;Hattar, Samer
通讯作者：
Hattar, Samer