Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms.
Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms.
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DOI：
10.1038/ncomms4256
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发表时间：
2014
影响因子：
16.6
通讯作者：
Shepard, Kenneth L.
中科院分区：
文献类型：
--
作者：
Bellin, Daniel L.;Sakhtah, Hassan;Rosenstein, Jacob K.;Levine, Peter M.;Thimot, Jordan;Emmett, Kevin;Dietrich, Lars E. P.;Shepard, Kenneth L.
Despite advances in monitoring spatiotemporal expression patterns of genes and proteins with fluorescent probes, direct detection of metabolites and small molecules remains challenging. A technique for spatially resolved detection of small molecules would benefit the study of redox-active metabolites produced by microbial biofilms, which can drastically affect colony development. Here we present an integrated circuit-based electrochemical sensing platform featuring an array of working electrodes and parallel potentiostat channels. “Images” over a 3.25 × 0.9 mm area can be captured with a diffusion-limited spatial resolution of 750 μm. We demonstrate that square wave voltammetry can be used to detect, identify, and quantify (for concentrations as low as 2.6 μM) four distinct redox-active metabolites called phenazines. We characterize phenazine production in both wild-type and mutant Pseudomonas aeruginosa PA14 colony biofilms, and find correlations with fluorescent reporter imaging of phenazine biosynthetic gene expression.
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影响因子：
2.8
作者：
Abu, Eme A.;Su, Shengchang;Hassett, Daniel J.
通讯作者：
Hassett, Daniel J.
影响因子：
3.6
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
通讯作者：
Newman, Dianne K.
DOI：
10.1073/pnas.1117298108
发表时间：
2011-12-13
影响因子：
11.1
作者：
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通讯作者：
Whiteley, Marvin
影响因子：
56.9
作者：
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通讯作者：
PRASHER, DC
影响因子：
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作者：
Kim, Eunkyoung;Gordonov, Tanya;Payne, Gregory F.
通讯作者：
Payne, Gregory F.