Electrophysiological differences between oxytocin and vasopressin neurones recorded from female rats in vitro.
Electrophysiological differences between oxytocin and vasopressin neurones recorded from female rats in vitro.
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体外记录的雌性大鼠催产素和加压素神经元之间的电生理差异。
DOI：
10.1113/jphysiol.1995.sp021001
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Armstrong,WE
中科院分区：
文献类型：
--
作者：
Stern,JE;Armstrong,WE
1.免疫化学鉴定的未交配或哺乳雌性大鼠视上核（SON）中催产素（OT）和加压素（VP）神经元的体外细胞内记录显示，当在静息膜电位下进行时，神经元类型之间在膜电位（Vm）、输入电阻和电流-电压关系（I-V）方面没有差异。2.当OT（94%），而不是VP，神经元（93%）在去极化电压（高于-50 mV）下被电流钳位时，小的超极化脉冲显示出时间和电压依赖性的外向整流，该整流存在于-75 mV以上，并且随着Vm接近钾的平衡电位（EK）而幅度降低。当神经元保持在更去极化的膜电位时，整流更明显，并且神经元保持去极化的时间越长，整流越大，在0.6 - 0.9 s时达到最大值。3.与整流相关的超极化脉冲的偏移之后是反弹去极化。反弹的峰值幅度表现出时间和电压依赖性。随着超极化命令变大，它遵循钟形曲线，在-65 +/-1.5 mV处达到峰值。反弹幅度随着脉冲持续时间的增加而增加，在0.5 +/-0.1 s时达到半最大幅度。4. OT神经元持续性外向整流和反跳的表达在处女和哺乳期雌性大鼠相似。5.这些结果表明，存在显着差异的内在膜特性，可能是K+电流，OT和VP神经元之间的哺乳期和处女雌性大鼠。
1. Intracellular recordings in vitro from immunochemically identified oxytocin (OT) and vasopressin (VP) neurones in the supraoptic nucleus (SON) of virgin or lactating female rats revealed no differences between neurone types in membrane potential (Vm), input resistance and current‐voltage relationships (I‐V), when taken at resting membrane potentials. 2. When OT (94%), but not VP, neurones (93%) were current clamped at depolarized voltages (above ‐50 mV), small hyperpolarizing pulses revealed a time‐ and voltage‐dependent outward rectification that was present above ‐75 mV and that decreased in amplitude as Vm approached the equilibrium potential for potassium (EK). The rectification was more pronounced when the neurones were held at a more depolarized membrane potential, and was larger the longer the neurone was held depolarized, reaching a maximum at 0.6‐0.9 s. 3. A rebound depolarization followed the offset of hyperpolarizing pulses that were associated with the rectification. The peak amplitude of the rebound showed a time and a voltage dependence. It followed a bell‐shaped curve as the hyperpolarizing commands were made larger, attaining a peak at ‐65 +/‐ 1.5 mV. The rebound amplitude increased with pulse duration, achieving a half‐maximal amplitude at 0.5 +/‐ 0.1 s. 4. The expression of the sustained outward rectification and the rebound in OT neurones was similar in virgin and lactating female rats. 5. These results indicate the presence of significant differences in the intrinsic membrane properties, probably K+ currents, between OT and VP neurones in both lactating and virgin female rats.
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影响因子：
2.9
作者：
Releasing Neurones;D. U. R. I. N. G. Progressive;N. Dehydratio;J. Wakerley;D. Poulain;D. Brown
通讯作者：
Releasing Neurones;D. U. R. I. N. G. Progressive;N. Dehydratio;J. Wakerley;D. Poulain;D. Brown
影响因子：
56.9
作者：
ANDREW, RD;DUDEK, FE
通讯作者：
DUDEK, FE
大鼠下丘脑视上核中的阶段性放电神经元：免疫细胞化学和电生理学研究
影响因子：
2.5
作者：
H. Yamashita;K. Inenaga;M. Kawata;Y. Sano
通讯作者：
Y. Sano
催产素和加压素分泌神经元的电生理分化
DOI：
--
发表时间：
1977
期刊：
Proceedings of the Royal Society of London. Series B. Biological Sciences
影响因子：
--
作者：
D. Poulain;J. Wakerley;R. Dyball
通讯作者：
R. Dyball
影响因子：
5.5
作者：
ANDREW, RD;DUDEK, FE
通讯作者：
DUDEK, FE