Streptococcus mutans Displays Altered Stress Responses While Enhancing Biofilm Formation by Lactobacillus casei in Mixed-Species Consortium.
Streptococcus mutans Displays Altered Stress Responses While Enhancing Biofilm Formation by Lactobacillus casei in Mixed-Species Consortium.
复制标题
DOI：
10.3389/fcimb.2017.00524
复制
发表时间：
2017
影响因子：
5.7
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Wen ZT;Liao S;Bitoun JP;De A;Jorgensen A;Feng S;Xu X;Chain PSG;Caufield PW;Koo H;Li Y
像变形链球菌一样，乳酸菌通常是从龋齿部位分离出来的，尽管它们在龋齿发展中的确切作用尚不清楚。本研究采用混合种模型分析了主要口腔乳酸菌群的生物膜形成，包括干酪乳杆菌、发酵乳杆菌、鼠李糖乳杆菌和唾液乳杆菌。salivarii和L. gasseri。结果表明，乳酸菌单独生长时不能形成良好的生物膜，但不同菌种间存在差异。与变形链球菌一起生长时，发酵乳杆菌和鼠李糖乳杆菌的生物膜形成量增加了2-log (P < 0.001)，而发酵乳杆菌的生物膜形成量减少了1-log （P < 0.001）。与野生型变形链球菌一起生长时，干酪乳杆菌的生物膜形成提高了~2-log，而与缺乏葡萄糖转移酶GtfB和粘附素P1的变形链球菌一起生长时，没有观察到这种影响。变形链球菌和干酪乳杆菌在双种培养中都增强了对酸杀灭的抵抗力，存活率增加了bbbb1 -log (P < 0.001)，但与各自的单种培养相比，暴露于过氧化氢后存活率急剧降低（P < 0.001）。当通过RNA-seq分析时，与单独生长的菌株相比，在与干酪乳杆菌双种突变链球菌中鉴定出超过134个基因上调或下调。上调的基因包括超氧化物歧化酶、NADH氧化酶和抗变菌素生物合成簇的成员。下调的基因包括GtfB和备选sigma因子SigX。这些结果进一步表明变形链球菌与口腔乳酸菌之间的相互作用具有物种特异性，并可能对该群落的蛀牙潜力产生重大影响。
Like Streptococcus mutans, lactobacilli are commonly isolated from carious sites, although their exact role in caries development remains unclear. This study used mixed-species models to analyze biofilm formation by major groups of oral lactobacilli, including L. casei, L. fermentum, L. rhamnosus, L. salivarius ssp. salivarius, and L. gasseri. The results showed that lactobacilli did not form good biofilms when grown alone, although differences existed between different species. When grown together with S. mutans, biofilm formation by L. gasseri and L. rhamnosus was increased by 2-log (P < 0.001), while biofilms by L. fermentum reduced by >1-log (P < 0.001). L. casei enhanced biofilm formation by ~2-log when grown with S. mutans wild-type, but no such effects were observed with S. mutans deficient of glucosyltransferase GtfB and adhesin P1. Both S. mutans and L. casei in dual-species enhanced resistance to acid killing with increases of survival rate by >1-log (P < 0.001), but drastically reduced the survival rates following exposure to hydrogen peroxide (P < 0.001), as compared to the respective mono-species cultures. When analyzed by RNA-seq, more than 134 genes were identified in S. mutans in dual-species with L. casei as either up- or down-regulated when compared to those grown alone. The up-regulated genes include those for superoxide dismutase, NADH oxidase, and members of the mutanobactin biosynthesis cluster. Among the down-regulated genes were those for GtfB and alternative sigma factor SigX. These results further suggest that interactions between S. mutans and oral lactobacilli are species-specific and may have significant impact on cariogenic potential of the community.
登录
查看更多内容
影响因子：
2.1
作者：
Bitoun JP;Nguyen AH;Fan Y;Burne RA;Wen ZT
通讯作者：
Wen ZT
影响因子：
3.6
作者：
Brady LJ;Maddocks SE;Larson MR;Forsgren N;Persson K;Deivanayagam CC;Jenkinson HF
通讯作者：
Jenkinson HF
影响因子：
3.1
作者：
Falsetta, Megan L.;Klein, Marlise I.;Koo, Hyun
通讯作者：
Koo, Hyun
影响因子：
4.4
作者：
Gregoire, S.;Xiao, J.;Koo, H.
通讯作者：
Koo, H.
影响因子：
--
作者：
Burne, R A;Zeng, L;Nascimento, M M
通讯作者：
Nascimento, M M