Transcriptional repressor Rex is involved in regulation of oxidative stress response and biofilm formation by Streptococcus mutans.
Transcriptional repressor Rex is involved in regulation of oxidative stress response and biofilm formation by Streptococcus mutans.
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DOI：
10.1111/j.1574-6968.2011.02293.x
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发表时间：
2011-07
影响因子：
2.1
通讯作者：
Wen ZT
中科院分区：
文献类型：
--
作者：
Bitoun JP;Nguyen AH;Fan Y;Burne RA;Wen ZT
转录抑制因子Rex参与了氧化还原电位对能量代谢和发酵生长的调节。变形链球菌是人类龋齿的主要病原体，它拥有一种编码蛋白质的基因，这种蛋白质与Rex蛋白家族成员高度相似。在这项研究中，我们发现rex缺乏损害了变形链球菌应对氧化应激和形成生物膜的能力。rex缺陷突变体在3天后积累的生物膜也比野生型菌株少，特别是在含蔗糖培养基中生长时，但产生的细胞外葡聚糖比亲本菌株多。rex缺乏导致基因转录发生实质性改变，包括与异源代谢、NAD+再生和氧化应激有关的基因转录。在上调的基因中有gtfC，它编码葡萄糖基转移酶C，一种主要负责合成不溶于水的葡聚糖的酶。这些结果表明，Rex在变形链球菌的氧化应激反应和生物膜形成中起重要作用。
The transcriptional repressor Rex has been implicated in regulation of energy metabolism and fermentative growth in response to redox potential. Streptococcus mutans, the primary causative agent of human dental caries, possesses a gene that encodes a protein with high similarity to members of the Rex family of proteins. In this study, we showed that Rex-deficiency compromised the ability of S. mutans to cope with oxidative stress and to form biofilms. The Rex-deficient mutant also accumulated less biofilm after 3-days than the wild-type strain, especially when grown in sucrose-containing medium, but produced more extracellular glucans than the parental strain. Rex-deficiency caused substantial alterations in gene transcription, including those involved in heterofermentative metabolism, NAD+ regeneration and oxidative stress. Among the up-regulated genes was gtfC, which encodes glucosyltransferase C, an enzyme primarily responsible for synthesis of water-insoluble glucans. These results reveal that Rex plays an important role in oxidative stress responses and biofilm formation by S. mutans.
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