Synthesis and functional studies of THF-gramicidin hybrid ion channels.
Synthesis and functional studies of THF-gramicidin hybrid ion channels.
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THF-短杆菌肽混合离子通道的合成和功能研究。
DOI：
10.1039/b303249n
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发表时间：
2003
影响因子：
3.2
通讯作者：
U. Koert
中科院分区：
文献类型：
--
作者：
A. Vescovi;Andrea Knoll;U. Koert
合成了11和12位为L-THF氨基酸1的短杆菌肽-短杆菌肽杂合物2-4和10和11位为D-THF氨基酸ent-1的短杆菌肽杂合物5-8，并通过单通道电流分析研究了它们的离子通道性质。用L-THF氨基酸1取代位置11和12得到强烈降低的通道性能。相反，取代位置10和11的D-THF氨基酸ent-1引起了新的和有趣的通道特性。对于渗透率比，离子选择性从Eisenman I向Eisenman III选择性转移，并且通道显示ms-动力学。最值得注意的是不对称化合物8，它选择性地插入DPhPC膜，并显示电压导向的门控动力学。
THF-gramicidin hybrids 2-4 with the L-THF amino acid 1 in positions 11 and 12 and compounds 5-8 with the D-THF amino acid ent-1 in positions 10 and 11 were synthesized and their ion channel properties were studied by single-channel-current analysis. The replacement of positions 11 and 12 by the L-THF amino acid 1 gave a strongly reduced channel performance. In contrast, replacement of positions 10 and 11 by the D-THF amino acid ent-1 gave rise to new and interesting channel properties. For the permeability ratios, the ion selectivity shifts from Eisenman I towards Eisenman III selectivity and the channels display ms-dynamics. Most remarkable is the asymmetric compound 8, which inserts selectively into a DPhPC membrane and displays voltage-directed gating dynamics.
影响因子：
56.9
作者：
KETCHEM, RR;HU, W;CROSS, TA
通讯作者：
CROSS, TA
短杆菌肽通道的设计和表征。
影响因子：
--
作者：
Greathouse,DV;Koeppe2nd,RE;Providence,LL;Shobana,S;Andersen,OS
通讯作者：
Andersen,OS
影响因子：
3.4
作者：
Akeson, M;Branton, D;Deamer, DW
通讯作者：
Deamer, DW