Transcriptional activation and translocation of ancient NOS during immune response
Transcriptional activation and translocation of ancient NOS during immune response
复制标题
免疫反应过程中古 NOS 的转录激活和易位
DOI：
10.1096/fj.201500193rr
复制
发表时间：
2016-10
期刊：
影响因子：
4.8
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Jiang Qiufen;Liu Zhaoqun;Zhou Zhi;Wang Lingling;Wang Leilei;Yue Feng;Wang Jingjing;Wang Hao;Song Linsheng
NOS是NO系统的关键组成部分，在许多生理和免疫过程中发挥着不可或缺的作用；然而，古代 NOS 激活的过程及其功能仍不清楚。用 LPS 和 TNF-α 刺激牡蛎后，检查了血细胞中巨牡蛎一氧化氮合酶 (CgNOS) mRNA 的表达。刺激后 24 小时，CgNOS mRNA 的表达水平显着增加 2.61 倍（P <0.05）。通过免疫沉淀检测到阳性 CgNOS 信号，并且在牡蛎血细胞中仅检测到一种蛋白质。在 CgNOS 启动子和转录因子 CgNF-κB1 以及 Cg 信号转导子和转录激活子 (STAT) 之间的 EMSA 中观察到移动和超移动带。仅在12小时时在细胞核中检测到CgNF-κB1，而在12小时和24小时时在细胞质和细胞核中观察到CgSTAT。在LPS和TNF-α刺激12小时后的转录组分析中，酪氨酸蛋白激酶受体Tie-1、磷脂酰肌醇磷酸酶SAC2、磷脂酰肌醇-4-磷酸5-激酶1a型、二酰甘油激酶u、LPS诱导的TNF-α因子样蛋白、cAMP依赖性转录因子-2、NF-κB1和STAT6的表达水平显着升高。 12小时时，在细胞膜和细胞质中均观察到免疫反应性CgNOS信号，而LPS和-TNF-α刺激后24小时，其主要定位于细胞质。这些发现表明，CgNOS 可以通过 PI3K-Akt 途径被 CgNF-κB1 和 CgSTAT 转录激活，类似于 iNOS 的情况，但 CgNOS 易位到细胞质，类似于神经元 NOS，在免疫防御过程中调节下游信号。这些结果共同提供了有关 NOS 结构和功能进化的重要知识。—Jiang, Q., Liu, Z., Zhou, Z., Wang, L., Wang, L., Yue, F., Wang, J., Wang, H., Song, L. 免疫反应过程中古 NOS 的转录激活和易位。 FASEB J. 30, 3527–3540 (2016)。 www.fasebj.org
NOS is the key component of the NO system, which plays an indispensable role in many physiologic and immunologic processes; however, the process that underlies the activation of ancient NOSs and their functions remains unclear. Expression of Crassostrea gigas NOS (CgNOS) mRNA in hemocytes was examined after stimulating oysters with LPS and TNF‐α. Expression level of CgNOS mRNA was increased significantly, by 2.61‐fold (P <0.05), at 24 h poststimulation. A positive CgNOS signal was detected via immunoprecipitation, and only one protein was detected in oyster hemocytes. Shifting and supershifting bands were observed in EMSAs between the CgNOS promoter and the transcription factors CgNF‐κB1 and Cg‐signal transducer and activator of transcription (STAT). CgNF‐κB1 was detected in the nucleus only at 12 h, whereas CgSTAT was observed in the cytoplasm and nucleus at 12 and 24 h. Expression levels of tyrosine‐protein kinase receptor Tie‐1, phosphatidylinositide phosphatase SAC2, phosphatidylinositol‐4‐phosphate 5‐kinase type‐1a, diacylglycerol kinase u, LPS‐induced TNF‐α factor‐like protein, cAMP‐dependent transcription factor‐2, NF‐κB1, and STAT6 were significantly elevated in a transcriptome analysis after 12 h of LPS and TNF‐α stimulation. An immunoreactive CgNOS signal was observed in both the cell membrane and cytoplasm at 12 h, whereas it was mainly localized to the cytoplasm at 24 h post‐LPS and –TNF‐α stimulation. These findings revealed that CgNOS could be transcriptionally activated by CgNF‐κB1 and CgSTAT via the PI3K‐Akt pathway, similar to what occurs for iNOS, but CgNOS translocated to the cytoplasm, similar to neuronal NOS, to modulate downstream signals during an immune defense. These results collectively provide crucial knowledge about the evolution of NOS structure and function.—Jiang, Q., Liu, Z., Zhou, Z., Wang, L., Wang, L., Yue, F., Wang, J., Wang, H., Song, L. Transcriptional activation and translocation of ancient NOS during immune response. FASEB J. 30, 3527–3540 (2016). www.fasebj.org
登录
查看更多内容
影响因子：
5.6
作者：
GILBERT, RS;HERSCHMAN, HR
通讯作者：
HERSCHMAN, HR
影响因子：
0.7
作者：
R. Barcia;JI Ramos-Martinez
通讯作者：
R. Barcia;JI Ramos-Martinez
通过数字基因表达分析，成功免疫反应在牡蛎crassostrea gigas中成功免疫反应的整体转录组分析。
影响因子：
3.7
作者：
de Lorgeril J;Zenagui R;Rosa RD;Piquemal D;Bachère E
通讯作者：
Bachère E
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
4.4
作者：
E. Chan;B. Winston;S. Uh;M. Wynes;D. M. Rose;D. Riches
通讯作者：
E. Chan;B. Winston;S. Uh;M. Wynes;D. M. Rose;D. Riches