Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis.
Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis.
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通过数字基因表达分析，成功免疫反应在牡蛎crassostrea gigas中成功免疫反应的整体转录组分析。
DOI：
10.1371/journal.pone.0023142
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Bachère E
中科院分区：
文献类型：
--
作者：
de Lorgeril J;Zenagui R;Rosa RD;Piquemal D;Bachère E
几十年来，由于环境参数、牡蛎生理和/或遗传状态以及包括弧菌在内的病原微生物的存在等因素的相互作用，养殖的太平洋牡蛎长牡蛎遭受了大规模的夏季死亡现象。为了全面了解巨噬线虫免疫应答以规避弧菌感染的分子机制，我们对免疫活性细胞血细胞的转录组进行了首次深度测序研究。利用数字基因表达技术（Digital Gene Expression， DGE），我们生成了一份转录本目录，比较了在毒弧菌（Vibrio脾弧菌LGP32和V. aestuarianus LPi 02/41）感染后存活的牡蛎与无毒弧菌塔斯马尼亚弧菌LMG 20012T感染后存活的牡蛎的上调基因。为此，开发了一种原始的实验感染方案，其中只有能够在感染中存活的动物才被考虑用于DGE方法。我们报告了细胞和免疫功能的鉴定，这些功能表征了牡蛎在致病性弧菌感染中存活的能力。功能注释突出了与免疫应答信号转导、细胞粘附和通讯、吞噬、肌动蛋白细胞骨架重组、细胞运输和自噬等细胞过程和防御机制以及抗氧化和抗凋亡反应相关的基因。此外，定量PCR分析首次发现了牡蛎基因调控中的病原特异性特征，为深入研究牡蛎与病原的相互作用及其发病机制开辟了道路。这项工作是确定控制牡蛎在弧菌感染中存活能力的生理特征的先决条件，随后是更好地理解夏季死亡现象的先决条件。
The cultivated Pacific oyster Crassostrea gigas has suffered for decades large scale summer mortality phenomenon resulting from the interaction between the environment parameters, the oyster physiological and/or genetic status and the presence of pathogenic microorganisms including Vibrio species. To obtain a general picture of the molecular mechanisms implicated in C. gigas immune responsiveness to circumvent Vibrio infections, we have developed the first deep sequencing study of the transcriptome of hemocytes, the immunocompetent cells. Using Digital Gene Expression (DGE), we generated a transcript catalog of up-regulated genes from oysters surviving infection with virulent Vibrio strains (Vibrio splendidus LGP32 and V. aestuarianus LPi 02/41) compared to an avirulent one, V. tasmaniensis LMG 20012T. For that an original experimental infection protocol was developed in which only animals that were able to survive infections were considered for the DGE approach. We report the identification of cellular and immune functions that characterize the oyster capability to survive pathogenic Vibrio infections. Functional annotations highlight genes related to signal transduction of immune response, cell adhesion and communication as well as cellular processes and defence mechanisms of phagocytosis, actin cytosqueleton reorganization, cell trafficking and autophagy, but also antioxidant and anti-apoptotic reactions. In addition, quantitative PCR analysis reveals the first identification of pathogen-specific signatures in oyster gene regulation, which opens the way for in depth molecular studies of oyster-pathogen interaction and pathogenesis. This work is a prerequisite for the identification of those physiological traits controlling oyster capacity to survive a Vibrio infection and, subsequently, for a better understanding of the phenomenon of summer mortality.
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影响因子：
2.9
作者：
Montagnani, C.;Avarre, J. C.;Escoubas, J. M.
通讯作者：
Escoubas, J. M.
影响因子：
3.6
作者：
Garnier, M.;Labreuche, Y.;Nicolas, J. -L.
通讯作者：
Nicolas, J. -L.
影响因子：
2.9
作者：
Guillou, Francois;Mitta, Guillaume;Coustau, Christine
通讯作者：
Coustau, Christine
影响因子：
2.9
作者：
Montagnani, C.;Labreuche, Y.;Escoubas, J. M.
通讯作者：
Escoubas, J. M.
影响因子：
30.3
作者：
Deretic V;Levine B
通讯作者：
Levine B