Regulation of caveolin-1 expression and phosphorylation by VEGF in ovine amnion cells.
Regulation of caveolin-1 expression and phosphorylation by VEGF in ovine amnion cells.
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DOI：
10.1177/1933719110378175
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Brace RA
中科院分区：
文献类型：
--
作者：
Cheung CY;Li S;Chen D;Brace RA
血管内皮生长因子（VEGF）已被牵连在羊水通过小窝跨羊膜的囊泡运输的调节。本研究验证了VEGF通过刺激小窝蛋白-1在绵羊羊膜上皮细胞（oAECs）中的表达和磷酸化来调节小窝功能的假设。使用oAEC的原代培养物，通过免疫荧光染色鉴定小窝蛋白-1。通过逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法测定小窝蛋白-1信使RNA（mRNA）丰度。测定VEGF 165对小窝蛋白-1表达和磷酸化的影响。Caveolin-1在oAECs中表达。响应于10 ng/mL VEGF 165，小窝蛋白-1 mRNA水平增加，而蛋白水平不受影响。此外，VEGF刺激小窝蛋白-1磷酸化，c-Src蛋白激酶的抑制废除的效果。这些数据表明，VEGF通过c-Src信号通路上调小窝蛋白-1的活性。我们的观察结果支持这一假设，即VEGF调节羊水运输通过羊膜刺激小窝蛋白-1活性介导的小窝功能在羊膜细胞。
Vascular endothelial growth factor (VEGF) has been implicated in the regulation of vesicular transport of amniotic fluid via caveolae across the amnion. This study tested the hypothesis that VEGF regulates caveolar function by stimulating caveolin-1 expression and phosphorylation in ovine amniotic epithelial cells (oAECs). Using primary cultures of oAECs, caveolin-1 was identified by immunofluorescent staining. Caveolin-1 messenger RNA (mRNA) abundance was determined by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and protein by Western blotting. The effects of VEGF165 on caveolin-1 expression and phosphorylation were determined. Caveolin-1 immunoreactivity was detected in oAECs. In response to 10 ng/mL VEGF165, caveolin-1 mRNA levels increased whereas the protein levels were unaffected. Furthermore, VEGF stimulated caveolin-1 phosphorylation, an effect abrogated by the inhibition of c-Src protein kinase. These data suggest that VEGF upregulates caveolin-1 activity through c-Src signaling pathways. Our observations support the hypothesis that VEGF regulates amniotic fluid transport across the amnion by stimulating caveolin-1 activity to mediate caveolar function in amnion cells.
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影响因子：
9.8
作者：
Gesteland, Katherine M.;Anderson, Debra F.;Brace, Robert A.
通讯作者：
Brace, Robert A.
影响因子：
3.8
作者：
Linton, EA;Rodriguez-Linares, B;Redman, CWG
通讯作者：
Redman, CWG
DOI：
10.1016/s1071-5576(99)00016-7
发表时间：
1999-07-01
期刊：
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
影响因子：
--
作者：
Cheung, CY;Brace, RA
通讯作者：
Brace, RA
影响因子：
4.8
作者：
Liu, J;Razani, B;Lisanti, MP
通讯作者：
Lisanti, MP
影响因子：
3.8
作者：
Lyden, TW;Anderson, CL;Robinson, JM
通讯作者：
Robinson, JM