Adaptive evolution of an industrial strain of Saccharomyces cerevisiae for combined tolerance to inhibitors and temperature.
Adaptive evolution of an industrial strain of Saccharomyces cerevisiae for combined tolerance to inhibitors and temperature.
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DOI：
10.1186/1754-6834-6-151
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发表时间：
2013-10-20
影响因子：
6.3
通讯作者：
Gorwa-Grauslund MF
中科院分区：
文献类型：
--
作者：
Wallace-Salinas V;Gorwa-Grauslund MF
开发对生物质预处理过程中释放的抑制剂具有高耐受性的工业酵母菌株对于生物乙醇生产至关重要。将这一特性与增加的耐热性相结合，将通过同时发酵和发酵（SSF）实现更有效的生产，并降低冷却成本。本工作的目的是开发一种结合这些特性的酵母菌株。采用长期适应策略，从工业菌株乙醇红（ER）中进化出稳定的酿酒酵母分离株（ISO 12）。与亲本菌株相反，ISO 12能够在39°C下生长和发酵未脱毒云杉水解产物的液体部分，乙醇产率为0.38g乙醇。g己糖-1。与以前的研究相比，ISO 12的上级表型不依赖于糠醛抑制剂转化的较高还原酶活性，而是依赖于较高的耐热性。与ER相比，ISO 12在39°C下发酵水解产物的能力更高，在52°C下热休克期间的活力更高。然而，在不存在抑制剂的情况下，ER和ISO 12在39°C下显示出相似的生长表型。进化分离ISO 12显示出上级的表型比亲本菌株ER时，两个应力，温度和抑制水解产物衍生的化合物，一起施加。结果表明，抑制剂的存在将允许生长的最高温度降低到39°C以下。由于适应过程和获得的耐热性改善，ISO 12能够克服这种协同效应。强健的菌株，如ISO 12，是SSF第二代乙醇生产的有趣候选者，以及在热带国家，在较高温度下发酵可以积极影响生产成本。
Development of industrial yeast strains with high tolerance towards the inhibitors released during biomass pretreatment is critical for bioethanol production. Combining this trait with increased thermotolerance would result in a more efficient production via Simultaneous Saccharification and Fermentation (SSF) as well as reduced cooling costs. The aim of the present work was to develop a yeast strain combining these traits. Using a long-term adaptation strategy a stable Saccharomyces cerevisiae isolate (ISO12) was evolved from the industrial strain Ethanol Red (ER). ISO12, contrary to the parental strain, is capable of growing and fermenting the liquid fraction of non-detoxified spruce hydrolysate at 39°C with an ethanol yield of 0.38 g ethanol . g hexoses-1. In contrast with previous studies, the superior phenotype of ISO12 does not rely on higher reductase activities for furaldehyde inhibitor conversion, but rather on a higher thermotolerance. ISO12 shows a higher capacity to ferment hydrolysate at 39°C and higher viability during heat-shock at 52°C than ER. In the absence of inhibitors, however, both ER and ISO12 displayed similar growth phenotype at 39°C. The evolved isolate ISO12 shows a superior phenotype than the parental strain ER when both stresses, temperature and inhibition by hydrolysate-derived compounds, are applied together. The results suggest that the presence of inhibitors depress the maximum temperature permissible for growth to a value below 39°C. As a result of the adaptation process and acquired improved thermotolerance, ISO12 is able to overcome this synergistic effect. Robust strains, such as ISO12, are interesting candidates for second generation ethanol production by SSF, as well as in tropical countries where fermentations at higher temperature can positively impact the production costs.
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影响因子：
9.8
作者：
Levy SF;Ziv N;Siegal ML
通讯作者：
Siegal ML
影响因子：
2.7
作者：
An, Ming-Zhe;Tang, Yue-Qin;Kenji, Kida
通讯作者：
Kenji, Kida
影响因子：
4.1
作者：
BANAT, IM;NIGAM, P;MARCHANT, R
通讯作者：
MARCHANT, R
DOI：
10.1007/s10295-004-0148-3
发表时间：
2004-09-01
影响因子：
3.4
作者：
Liu, ZL;Slininger, PJ;Gorsich, SW
通讯作者：
Gorsich, SW
影响因子：
5
作者：
Liu, Z. Lewis;Moon, Jaewoong;Weber, Scott
通讯作者：
Weber, Scott