Promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton FLOWERING LOCUS T (FT)-like gene GhFT1.
Promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton FLOWERING LOCUS T (FT)-like gene GhFT1.
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促进过表达棉花 FLOWERING LOCUS T (FT) 样基因 GhFT1 的烟草植物的开花、侧枝生长、叶片发育和花脱落
DOI：
10.3389/fpls.2015.00454
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发表时间：
2015
影响因子：
5.6
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Li C;Zhang Y;Zhang K;Guo D;Cui B;Wang X;Huang X
FLOWERLOCUS T（FT）编码一个移动的信号蛋白，是成花素的主要成分，在植物成花调控中具有重要的地位，在许多生理方面也发挥着重要作用。最近出现了一种模式的平衡，无限和有限的增长，这是控制的比例FT-样和MATERIAL FLOWER 1（TFL 1）-样基因的活动，并有很大的影响，花的过渡和植物的建筑。GhFT 1的直系同源物先前从陆地棉中分离并表征。结果表明，GhFT 1基因在烟草中的异位过表达，除了促进开花外，还促进了基部侧枝的生长，诱导了莲座叶腋部腋芽的产生，改变了叶片形态，增加了叶绿素含量，提高了光合速率，并导致花朵脱落。基因表达分析表明，花的身份基因显着上调转基因植物。进一步分析烟草FT旁系同源物表明，NtFT 4，作为花诱导剂，上调，而NtFT 2和NtFT 3作为花抑制剂上调，在转基因植物在长日照条件下，但在短日照条件下下调。我们的数据表明，足够水平的转基因棉花FT可能会扰乱内源烟草FT旁系同源物的诱导子和阻遏子的平衡，并导致转基因烟草的表型改变，强调FT在调节地上部结构通过推进确定的生长扩大的作用。在整个FT样和TFL 1样基因活性中操纵不确定和确定生长因子的比例有望改善植物结构并提高作物产量。
FLOWERING LOCUS T (FT) encodes a mobile signal protein, recognized as major component of florigen, which has a central position in regulating flowering, and also plays important roles in various physiological aspects. A mode is recently emerging for the balance of indeterminate and determinate growth, which is controlled by the ratio of FT-like and TERMINAL FLOWER 1 (TFL1)-like gene activities, and has a strong influence on the floral transition and plant architecture. Orthologs of GhFT1 was previously isolated and characterized from Gossypium hirsutum. We demonstrated that ectopic overexpression of GhFT1 in tobacco, other than promoting flowering, promoted lateral shoot outgrowth at the base, induced more axillary bud at the axillae of rosette leaves, altered leaf morphology, increased chlorophyll content, had higher rate of photosynthesis and caused flowers abscission. Analysis of gene expression suggested that flower identity genes were significantly upregulated in transgenic plants. Further analysis of tobacco FT paralogs indicated that NtFT4, acting as flower inducer, was upregulated, whereas NtFT2 and NtFT3 as flower inhibitors were upregulated in transgenic plants under long-day conditions, but downregulated under short-day conditions. Our data suggests that sufficient level of transgenic cotton FT might disturb the balance of the endogenous tobacco FT paralogs of inducers and repressors and resulted in altered phenotype in transgenic tobacco, emphasizing the expanding roles of FT in regulating shoot architecture by advancing determine growth. Manipulating the ratio for indeterminate and determinate growth factors throughout FT-like and TFL1-like gene activity holds promise to improve plant architecture and enhance crop yield.
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影响因子：
5.3
作者：
Ikegami H;Nogata H;Inoue Y;Himeno S;Yakushiji H;Hirata C;Hirashima K;Mori M;Awamura M;Nakahara T
通讯作者：
Nakahara T
影响因子：
11.6
作者：
Hayama, Ryosuke;Agashe, Bhavna;Coupland, George
通讯作者：
Coupland, George
影响因子：
5.1
作者：
Jose Carmona, Maria;Calonje, Myriam;Miguel Martinez-Zapater, Jose
通讯作者：
Miguel Martinez-Zapater, Jose
影响因子：
4.3
作者：
Fukuda, Machiko;Matsuo, Satoshi;Honda, Ichiro
通讯作者：
Honda, Ichiro
影响因子：
11.6
作者：
Hsu, Chuan-Yu;Liu, Yunxia;Yuceer, Cetin
通讯作者：
Yuceer, Cetin