Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells.
Matrin-3 is essential for fibroblast growth factor 2-dependent maintenance of neural stem cells.
复制标题
DOI：
10.1038/s41598-018-31597-x
复制
发表时间：
2018-09-07
影响因子：
4.6
通讯作者：
Niimori D
中科院分区：
文献类型：
--
作者：
Niimori-Kita K;Tamamaki N;Koizumi D;Niimori D
为了研究神经干细胞维持的机制，我们进行了针对核磷酸化蛋白的二维荧光差示凝胶电泳法(2D-DGE)。在成纤维细胞生长因子2(FGF2)刺激下，神经干细胞(NSCs)中检测到核磷酸化蛋白Matrin-3。Matrin-3在小鼠胚胎脑室下区和脑室区有表达。小干扰RNA(SiRNA)介导的Matrin-3基因敲除可在体外诱导NSCs向神经元分化，并在体内改变胎脑的脑层结构。将丝氨酸208位点突变为丙氨酸的Matrin-3质粒(Ser208Ala突变体Matrin3)和抑制可磷酸化Matrin-3 Ser208残基的毛细血管扩张共济失调突变激酶(ATM Kinase)，可诱导神经元分化，并抑制神经球形成干细胞的增殖。因此，我们的蛋白质组学方法表明，Matrin-3的磷酸化对于体外和体内依赖FGF2的神经干细胞的维持是必不可少的。
To investigate the mechanisms underlying the maintenance of neural stem cells, we performed two-dimensional fluorescence-difference gel electrophoresis (2D-DIGE) targeting the nuclear phosphorylated proteins. Nuclear phosphorylated protein Matrin-3 was identified in neural stem cells (NSCs) after stimulation using fibroblast growth factor 2 (FGF2). Matrin-3 was expressed in the mouse embryonic subventricular and ventricular zones. Small interfering RNA (siRNA)-mediated knockdown of Matrin-3 caused neuronal differentiation of NSCs in vitro, and altered the cerebral layer structure of foetal brain in vivo. Transfection of Matrin-3 plasmids in which the serine 208 residue was point-mutated to alanine (Ser208Ala mutant Matrin3) and inhibition of Ataxia telangiectasia mutated kinase (ATM kinase), which phosphorylates Matrin-3 Ser208 residue, caused neuronal differentiation and decreased the proliferation of neurosphere-forming stem cells. Thus, our proteomic approach revealed that Matrin-3 phosphorylation was essential for FGF2-dependent maintenance of NSCs in vitro and in vivo.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2006.12.191
发表时间：
2007-03-02
影响因子：
3.1
作者：
Hisada-Ishii, Shoji;Ebihara, Mizuki;Kitagawa, Yasuo
通讯作者：
Kitagawa, Yasuo
影响因子：
4.8
作者：
Biton, Sharon;Dar, Inbal;Shiloh, Yosef
通讯作者：
Shiloh, Yosef
影响因子：
5.3
作者：
Fukuda, Shinji;Abematsu, Masahiko;Taga, Tetsuya
通讯作者：
Taga, Tetsuya
影响因子：
5.2
作者：
Levenstein, Mark E.;Ludwig, Tenneille E.;Thomson, James A.
通讯作者：
Thomson, James A.
影响因子：
25
作者：
Johnson, Janel O.;Pioro, Erik P.;Boehringer, Ashley;Chia, Ruth;Feit, Howard;Renton, Alan E.;Pliner, Hannah A.;Abramzon, Yevgeniya;Marangi, Giuseppe;Winborn, Brett J.;Gibbs, J. Raphael;Nalls, Michael A.;Morgan, Sarah;Shoai, Maryam;Hardy, John;Pittman, Alan;Orrell, Richard W.;Malaspina, Andrea;Sidle, Katie C.;Fratta, Pietro;Harms, Matthew B.;Baloh, Robert H.;Pestronk, Alan;Weihl, Conrad C.;Rogaeva, Ekaterina;Zinman, Lorne;Drory, Vivian E.;Borghero, Giuseppe;Mora, Gabriele;Calvo, Andrea;Rothstein, Jeffrey D.;Drepper, Carsten;Sendtner, Michael;Singleton, Andrew B.;Taylor, J. Paul;Cookson, Mark R.;Restagno, Gabriella;Sabatelli, Mario;Bowser, Robert;Chio, Adriano;Traynor, Bryan J.
通讯作者：
Traynor, Bryan J.