Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells
Proteomic identification of differently expressed proteins responsible for osteoblast differentiation from human mesenchymal stem cells
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蛋白质组学鉴定负责人间充质干细胞成骨细胞分化的不同表达蛋白
DOI：
10.1007/s11010-007-9497-3
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发表时间：
2007-05
影响因子：
4.3
通讯作者：
中科院分区：
文献类型：
--
作者：
人间充质干细胞（hMSC）是一群多能细胞，其可以分化成成骨细胞、软骨细胞、脂肪细胞和其他细胞。hMSC向成骨细胞分化的确切机制在很大程度上仍不清楚。在这里，我们分析了蛋白质表达谱的未分化以及成骨诱导的hMSC使用2-D凝胶电泳（2-DE），质谱（MS），和肽质量指纹（PMF）调查成骨细胞分化的早期基因表达。我们已经生成了未分化的hMSC和成骨诱导的hMSC在第3天和第7天的蛋白质组图谱。2-DE共检测到102个蛋白质点，其中52个蛋白质点经MALDI-TOF-MS鉴定为差异表达蛋白，这些蛋白质可分为代谢、信号转导、转录、钙结合蛋白、蛋白质降解、蛋白质折叠和其他7类。通过进一步的RT-PCR分析证实了一些鉴定的蛋白质的表达。本研究阐明了成骨细胞分化过程中的整体蛋白质组。我们的研究结果将在更好地阐明hMSC向成骨细胞分化的分子机制中发挥重要作用。
Human mesenchymal stem cells (hMSC) are a population of multipotent cells that can differentiate into osteoblasts, chondrocytes, adipocytes, and other cells. The exact mechanism governing the differentiation of hMSC into osteoblasts remains largely unknown. Here, we analyzed protein expression profiles of undifferentiated as well as osteogenic induced hMSC using 2-D gel electrophoresis (2-DE), mass spectrometry (MS), and peptide mass fingerprinting (PMF) to investigate the early gene expression in osteoblast differentiation. We have generated proteome maps of undifferentiated hMSC and osteogenic induced hMSC on day 3 and day 7. 2-DE revealed 102 spots with at least 2.0-fold changes in expression and 52 differently expressed proteins were successfully identified by MALDI-TOF-MS. These proteins were classified into 7 functional categories: metabolism, signal transduction, transcription, calcium-binding protein, protein degradation, protein folding and others. The expression of some identified proteins was confirmed by further RT-PCR analyses. This study clarifies the global proteome during osteoblast differentiation. Our results will play an important role in better elucidating the underlying molecular mechanism in hMSC differentiation into osteoblasts.
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影响因子：
4.4
作者：
Yang, JW;Rodrigo, R;Lubec, G
通讯作者：
Lubec, G
影响因子：
8.4
作者：
Loeffler-Ragg, J;Skvortsov, S;Zwierzina, H
通讯作者：
Zwierzina, H
DOI：
10.1016/s0006-291x(03)00487-x
发表时间：
2003-04
影响因子：
3.1
作者：
R. Stilo;D. Liguoro;B. Jeso;A. Leonardi;P. Vito
通讯作者：
R. Stilo;D. Liguoro;B. Jeso;A. Leonardi;P. Vito
DOI：
10.1073/pnas.0532693100
发表时间：
2003-03-18
影响因子：
11.1
作者：
Qi, HL;Aguiar, DJ;Verfaillie, CM
通讯作者：
Verfaillie, CM
DOI：
10.1006/bbrc.2001.6196
发表时间：
2002-01-11
影响因子：
3.1
作者：
Doi, M;Nagano, A;Nakamura, Y
通讯作者：
Nakamura, Y