Profiles of acute cytokine and antibody responses in patients infected with avian influenza A H7N9.
Profiles of acute cytokine and antibody responses in patients infected with avian influenza A H7N9.
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DOI：
10.1371/journal.pone.0101788
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wu C
中科院分区：
文献类型：
--
作者：
Huang R;Zhang L;Gu Q;Zhou YH;Hao Y;Zhang K;Liu Y;Dong D;Wang S;Huang Z;Lu S;Wu C
2013年春季在中国东部爆发的甲型H7N9流感病毒是一种新型的、新兴的禽流感传播给人类。虽然H7N9感染的临床和微生物学特征已在文献中报道，但本研究调查了急性H7N9感染中的急性细胞因子和抗体反应。2013年3月27日至2013年4月23日，中国南京鼓楼医院收治了6例确诊的H7N9流感感染患者。急性期血清细胞因子谱使用高通量多重测定法测定。通过ELISA监测每日H7血凝素（HA）特异性IgG、IgM和伊加应答。针对表达新型H7亚型HA抗原的假型病毒测定H7N9病毒特异性中和抗体。与健康志愿者相比，H7N9感染患者中的五种细胞因子（IL-6，IP-10，IL-10，IFNγ和TNFα）显著升高。血清H7 HA特异性IgG，以及IgM和伊加反应，在疾病发作的8天内检测到，并在急性感染期间以类似的模式增加。中和抗体在结合抗体应答出现后不久产生，并且作为总H7 HA特异性IgG应答的一部分显示出相似的动力学。H7N9感染导致标志性血清细胞因子增加，这与发热和疾病持续相关。在急性H7N9感染中同时产生IgG、IgM和伊加反应的新发现表明，活流感病毒有可能引发快速和有效的保护性抗体，以限制感染。
The influenza A H7N9 virus outbreak in Eastern China in the spring of 2013 represented a novel, emerging avian influenza transmission to humans. While clinical and microbiological features of H7N9 infection have been reported in the literature, the current study investigated acute cytokine and antibody responses in acute H7N9 infection. Between March 27, 2013 and April 23, 2013, six patients with confirmed H7N9 influenza infection were admitted to Drum Tower Hospital, Nanjing, China. Acute phase serum cytokine profiles were determined using a high-throughput multiplex assay. Daily H7 hemagglutinin (HA)-specific IgG, IgM, and IgA responses were monitored by ELISA. Neutralizing antibodies specific for H7N9 viruses were determined against a pseudotyped virus expressing the novel H7 subtype HA antigen. Five cytokines (IL-6, IP-10, IL-10, IFNγ, and TNFα) were significantly elevated in H7N9-infected patients when compared to healthy volunteers. Serum H7 HA-specific IgG, as well as IgM and IgA responses, were detected within 8 days of disease onset and increased in a similar pattern during acute infection. Neutralizing antibodies developed shortly after the appearance of binding antibody responses and showed similar kinetics as a fraction of the total H7 HA-specific IgG responses. H7N9 infection resulted in hallmark serum cytokine increases, which correlated with fever and disease persistence. The novel finding of simultaneous development of IgG, IgM, and IgA responses in acute H7N9 infection points to the potential for live influenza viruses to elicit fast and potent protective antibodies to limit the infection.
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