Synthesis and characterization of nano ceria for biological applications

Synthesis and characterization of nano ceria for biological applications
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用于生物应用的纳米二氧化铈的合成和表征

DOI:
10.1117/12.2018564
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发表时间:
2013
期刊:
--
影响因子:
--
通讯作者:
Chen H
Chen H
中科院分区:
--
文献类型:
--
作者:
Chen H

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为了避免可能的细胞毒性,我们在没有任何添加剂的情况下通过铈盐和氢氧化钠反应合成了二氧化铈纳米颗粒。X射线衍射分析表明,所合成的氧化铈纳米粒子具有立方结构,透射电镜分析表明,其粒径在6 ~ 10 nm之间。用动态光散射法研究了二氧化铈纳米粒子在去离子水和细胞培养液中的聚集行为。在去离子水中,二氧化铈纳米颗粒形成稳定的胶体溶液,流体动力学尺寸约为70 nm,zeta电位约为47 mV。发现纳米颗粒在无血清介质中的分散导致更大的聚集体,而在含血清介质中的纳米颗粒形成稳定的溶液,这表明血清中的蛋白质和二氧化铈纳米颗粒具有一定的相互作用。在细胞摄取测试中,观察到二氧化铈纳米颗粒进入在含血清培养基中在37 °C下孵育60分钟的HeLa细胞中,并且没有细胞毒性。
We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria nanoparticles formed a stable colloidal solution with hydrodynamic size ~ 70 nm and zeta potential ~ 47 mV. Dispersion of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serumcontaining media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at 37 °C for 60 min was observed and without cytotoxicity.
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