Synthesis and characterization of nano ceria for biological applications
Synthesis and characterization of nano ceria for biological applications
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用于生物应用的纳米二氧化铈的合成和表征
DOI:
10.1117/12.2018564
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发表时间:
2013
期刊:
影响因子:
--
通讯作者:
Chen H
中科院分区:
文献类型:
--
作者:
Chen H
We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria nanoparticles formed a stable colloidal solution with hydrodynamic size ~ 70 nm and zeta potential ~ 47 mV. Dispersion of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serumcontaining media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at 37 °C for 60 min was observed and without cytotoxicity.
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DOI:
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发表时间:
2009
期刊:
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