Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane.
Wnt11 controls cell contact persistence by local accumulation of Frizzled 7 at the plasma membrane.
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DOI：
10.1083/jcb.200606017
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发表时间：
2006-12-04
期刊：
影响因子：
--
通讯作者：
Heisenberg CP
中科院分区：
文献类型：
--
作者：
Witzel S;Zimyanin V;Carreira-Barbosa F;Tada M;Heisenberg CP
Wnt 11是脊椎动物原肠胚形成过程中决定细胞极化和迁移的关键信号。已知Wnt 11在功能上与几种信号传导组分相互作用，这些信号传导组分的同源物控制果蝇中的平面细胞极性。虽然在D.黑腹动物这些成分被认为通过不对称地定位在质膜上使细胞极化，但目前尚不清楚它们的亚细胞定位是否在脊椎动物中发挥同样重要的作用。我们发现，在斑马鱼胚胎细胞中，Wnt 11通过在细胞接触的相邻位点上积累其受体Frizzled 7而在质膜上局部发挥作用。Wnt 11诱导的卷曲7积累招募细胞内Wnt信号传导介质Dishevelled，以及Wnt 11本身，并局部增加细胞接触持久性。这种细胞接触持久性的增加是由Wnt 11，Frizzled 7和质膜上的非典型钙粘蛋白Flamingo的局部相互作用介导的，并且它不需要Wnt 11信号传导的进一步下游效应子（如RhoA和Rok2）的活性。我们建议，Wnt 11，通过与卷曲7和火烈鸟，调制局部细胞接触持久性，以协调原肠胚形成过程中的细胞运动。
Wnt11 is a key signal, determining cell polarization and migration during vertebrate gastrulation. It is known that Wnt11 functionally interacts with several signaling components, the homologues of which control planar cell polarity in Drosophila melanogaster. Although in D. melanogaster these components are thought to polarize cells by asymmetrically localizing at the plasma membrane, it is not yet clear whether their subcellular localization plays a similarly important role in vertebrates. We show that in zebrafish embryonic cells, Wnt11 locally functions at the plasma membrane by accumulating its receptor, Frizzled 7, on adjacent sites of cell contacts. Wnt11-induced Frizzled 7 accumulations recruit the intracellular Wnt signaling mediator Dishevelled, as well as Wnt11 itself, and locally increase cell contact persistence. This increase in cell contact persistence is mediated by the local interaction of Wnt11, Frizzled 7, and the atypical cadherin Flamingo at the plasma membrane, and it does not require the activity of further downstream effectors of Wnt11 signaling, such as RhoA and Rok2. We propose that Wnt11, by interacting with Frizzled 7 and Flamingo, modulates local cell contact persistence to coordinate cell movements during gastrulation.
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DOI：
10.1083/jcb.133.5.1123
发表时间：
1996-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Torres MA;Yang-Snyder JA;Purcell SM;DeMarais AA;McGrew LL;Moon RT
通讯作者：
Moon RT
影响因子：
9.2
作者：
Montero, JA;Kilian, B;Heisenberg, CP
通讯作者：
Heisenberg, CP
影响因子：
16
作者：
Strutt, DI
通讯作者：
Strutt, DI
影响因子：
2.7
作者：
THISSE, C;THISSE, B;POSTLETHWAIT, JH
通讯作者：
POSTLETHWAIT, JH
影响因子：
9.2
作者：
Park, TJ;Gray, RS;Wallingford, JB
通讯作者：
Wallingford, JB