Tissue extracellular matrix nanoparticle presentation in electrospun nanofibers.
Tissue extracellular matrix nanoparticle presentation in electrospun nanofibers.
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DOI：
10.1155/2014/469120
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发表时间：
2014
影响因子：
--
通讯作者：
Elisseeff J
中科院分区：
文献类型：
--
作者：
Gibson M;Beachley V;Coburn J;Bandinelli PA;Mao HQ;Elisseeff J
来自成熟组织脱细胞化的生物材料保留了深刻影响细胞活性的生物学和结构特征。然而，这种材料的临床应用仍然有限，因为形状和物理性质难以控制。相比之下，基于合成聚合物的支架可以被工程化以表现出特定的物理性质，但通常受到有限的生物功能的影响。本研究表征了将脱细胞细胞外基质（DECM）颗粒与合成纳米纤维结合的复合材料，并研究了这些材料影响干细胞分化的能力。脱细胞组织的机械处理产生直径范围为71至334 nm的颗粒。设计并评价含有源自六种不同组织的高达10%DECM颗粒（wt/wt）的纳米纤维支架，以确认DECM颗粒掺入并测量生物活性。与对照组相比，含有骨、软骨和脂肪的支架在1周和3周促进了骨生成。相反，与对照组相比，脾和肺DECM显著降低了成骨结局。这些发现突出了将适当的源DECM纳米颗粒掺入纳米复合材料中以设计具有针对特定应用的生物活性的支架的潜力。
Biomaterials derived from the decellularization of mature tissues retain biological and architectural features that profoundly influence cellular activity. However, the clinical utility of such materials remains limited as the shape and physical properties are difficult to control. In contrast, scaffolds based on synthetic polymers can be engineered to exhibit specific physical properties, yet often suffer from limited biological functionality. This study characterizes composite materials that present decellularized extracellular matrix (DECM) particles in combination with synthetic nanofibers and examines the ability of these materials to influence stem cell differentiation. Mechanical processing of decellularized tissues yielded particles with diameters ranging from 71 to 334 nm. Nanofiber scaffolds containing up to 10% DECM particles (wt/wt) derived from six different tissues were engineered and evaluated to confirm DECM particle incorporation and to measure bioactivity. Scaffolds containing bone, cartilage, and fat promoted osteogenesis at 1 and 3 weeks compared to controls. In contrast, spleen and lung DECM significantly reduced osteogenic outcomes compared to controls. These findings highlight the potential to incorporate appropriate source DECM nanoparticles within nanofiber composites to design a scaffold with bioactivity targeted to specific applications.
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DOI：
10.1163/156856208784089599
发表时间：
2008-01-01
影响因子：
3.6
作者：
Stankus, John J.;Freytes, Donald O.;Wagner, William R.
通讯作者：
Wagner, William R.
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
10.8
作者：
Luu, YK;Kim, K;Hadjiargyrou, M
通讯作者：
Hadjiargyrou, M
影响因子：
2.8
作者：
Chen, Chia-Chun;Liao, Cheng-Hao;Fang, Hsu-Wei
通讯作者：
Fang, Hsu-Wei
DOI：
10.1074/mcp.m111.014647
发表时间：
2012-04
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Naba A;Clauser KR;Hoersch S;Liu H;Carr SA;Hynes RO
通讯作者：
Hynes RO