Transcriptomics analysis reveals candidate genes and pathways for susceptibility or resistance to Singapore grouper iridovirus in orange-spotted grouper (Epinephelus coioides).
Transcriptomics analysis reveals candidate genes and pathways for susceptibility or resistance to Singapore grouper iridovirus in orange-spotted grouper (Epinephelus coioides).
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转录组学分析揭示了橙斑石斑鱼（斜带石斑鱼）对新加坡石斑鱼虹彩病毒的易感性或抗性的候选基因和途径
DOI：
10.1016/j.dci.2018.09.003
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发表时间：
2019-01
影响因子：
2.9
通讯作者：
Qin Q
中科院分区：
文献类型：
--
作者：
Yang M;Wang Q;Wang S;Wang Y;Zeng Q;Qin Q
在这项研究中，石斑鱼的转录反应新加坡石斑虹彩病毒（SGIV）刺激的特点是使用RNA测序。使用Illumina MiSeq平台进行石斑鱼中三个测试组的转录组测序。三个测试组是对照组，其注射PBS缓冲液;高敏感（HS）组，其在SGIV注射后不久死亡;以及高抗性（HR）组，其在SGIV注射后存活。总共产生了38，253个unigenes。当HS组与对照组相比，885 unigenes上调和487 unigenes下调。当HR组和对照组进行比较时，1114个unigenes上调，420个下调，当HR组和HS组进行比较时，1010个unigenes上调，375个下调。在KEGG分析中，两个免疫相关的途径，p53和过氧化物酶体增殖物激活受体途径，被检测到具有高度显着的富集。此外，从我们的转录组数据中确定了7465个微卫星和22,1569个候选单核苷酸多态性。这些结果提示了与疾病易感性或抗病性性状相关的几种途径，并提供了关于新基因序列、基因表达谱和遗传标记的广泛信息。这可能有助于石斑鱼SGIV感染的疫苗研究和育种计划。本研究提出了石斑鱼抗病性和感病性相关的几条途径。本研究为石斑鱼新的基因序列、基因表达谱和遗传标记提供了信息。这可能有助于石斑鱼SGIV感染的疫苗研究和育种计划。
In this study, the transcriptional response of grouper to Singapore grouper iridovirus (SGIV) stimulation was characterized using RNA sequencing. Transcriptome sequencing of three test groups in the grouper was performed using the Illumina MiSeq platform. The three test groups were a control group, which was injected with PBS buffer; a high-susceptible (HS) group, which died shortly after the SGIV injection; and a high-resistance (HR) group, which survived the SGIV injection. In total, 38,253 unigenes were generated. When the HS group was compared with the control group, 885 unigenes were upregulated and 487 unigenes were downregulated. When the HR and control groups were compared, 1114 unigenes were upregulated and 420 were downregulated, and when the HR and HS groups were compared, 1010 unigenes were upregulated and 375 were downregulated. In the KEGG analysis, two immune-related pathways, the p53 and peroxisome proliferator-activated receptor pathways, were detected with highly significant enrichment. In addition, 7465 microsatellites and 22,1569 candidate single nucleotide polymorphisms were identified from our transcriptome data. The results suggested several pathways that are associated with traits of disease susceptibility or disease resistance, and provided extensive information about novel gene sequences, gene expression profiles, and genetic markers. This may contribute to vaccine research and a breeding program against SGIV infection in grouper. This study suggested several pathways associated with traits of disease susceptibility or disease resistance in grouper. This study offered information about novel gene sequences, gene expression profiles, and genetic markers in grouper. This may contribute to vaccine research and a breeding program against SGIV infection in grouper.
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DOI：
10.1161/01.atv.0000227472.70734.ad
发表时间：
2006-08-01
影响因子：
8.7
作者：
Han, Chang Yeop;Chiba, Tsuyoshi;Chait, Alan
通讯作者：
Chait, Alan
影响因子：
4
作者：
Delerive, P;Fruchart, JC;Staels, B
通讯作者：
Staels, B
DOI：
10.1038/nrmicro2071
发表时间：
2009-02
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
新加坡石斑鱼虹彩病毒是一种大型 DNA 病毒，通过细胞类型依赖性方式诱导非凋亡细胞死亡，并激发 ERK 信号传导
影响因子：
7.2
作者：
Huang, Xiaohong;Huang, Youhua;Qin, Qiwei
通讯作者：
Qin, Qiwei
DOI：
10.1016/j.bbrc.2003.09.185
发表时间：
2003-11-07
影响因子：
3.1
作者：
Hansmannel, F;Clémencet, MC;Nicolas-Francés, V
通讯作者：
Nicolas-Francés, V