Selenium Deficiency Facilitates Inflammation Through the Regulation of TLR4 and TLR4-Related Signaling Pathways in the Mice Uterus
Selenium Deficiency Facilitates Inflammation Through the Regulation of TLR4 and TLR4-Related Signaling Pathways in the Mice Uterus
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缺硒通过调节小鼠子宫中的 TLR4 和 TLR4 相关信号通路促进炎症
DOI：
10.1007/s10753-014-0106-9
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发表时间：
2015-01
期刊：
影响因子：
5.1
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
--
作者：
Wang, Tiancheng;Song, Xiaojing;Guo, Mengyao;Zhang, Naisheng
硒（Se）是一种重要的微量营养元素，影响生殖系统的发育和功能。子宫内膜炎是一种严重影响动物繁殖能力的生殖障碍性疾病。为研究硒缺乏对脂多糖（LPS）诱导的小鼠肠炎的影响，建立了LPS诱导的小鼠肠炎模型。用荧光分光光度法测定子宫组织中硒的含量。采用酶联免疫吸附试验（ELISA）和实时定量聚合酶链反应（qRT-PCR）测定肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和IL-6水平。Western blot检测IκBα、NF-κB p65、ERK、JNK和p38的磷酸化程度及Toll样受体4（TLR 4）的表达。用qRT-PCR分析TLR 4信使RNA（mRNA）。结果表明，日粮硒摄入量显著影响子宫组织硒水平。结果表明，低硒可导致LPS诱导的小鼠子宫内膜炎的病理改变，TNF-α、IL-1β、IL-6的表达增加，TLR 4的活化增强，IκBα、p65、ERK、JNK、p38的磷酸化水平升高。补充硒可抑制上述作用。总之，我们的研究表明，硒缺乏使小鼠子宫更容易发生炎症。适当补硒可提高子宫免疫功能。
Selenium (Se) is an essential nutritional trace element that affects the development and function of the reproductive system. Endometritis is a reproductive obstacle disease that can seriously reduce the reproductive capacity of animal. To study the effects of dietary Se deficiency on lipopolysaccharide (LPS)-induced mice endometritis, we generated a model of LPS-induced mice endometritis. The Se content in uterine tissues was detected by fluorescence spectrophotometry. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). The extent of phosphorylation of IκBα, NF-κB p65, ERK, JNK, and p38 and the expression of Toll-like receptor 4 (TLR4) were detected with Western blots. The TLR4 messenger RNA (mRNA) was analyzed with qRT-PCR. The results indicated that dietary Se intake significantly influenced Se levels in uterine tissues. The Se-deficient mice model was successfully replicated, and Se deficiency exacerbated uterine tissue histopathology; increased the expression of TNF-α, IL-1β, and IL-6; facilitated the activation of TLR4; and enhanced the phosphorylation of IκBα, p65, ERK, JNK, and p38 in LPS-induced mice endometritis. Also, the effects were inhibited by a supplement of Se. In conclusion, our studies demonstrated that Se deficiency makes mice uterus more prone to inflammation. An appropriate Se supplement could enhance the immune condition of the uterus.
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影响因子：
--
作者：
C. A. Dinarello
通讯作者：
C. A. Dinarello
DOI：
10.3181/00379727-182-42361
发表时间：
1986-09
影响因子：
--
作者：
J. Marsh;G. Combs;M. Whitacre;R. Dietert
通讯作者：
J. Marsh;G. Combs;M. Whitacre;R. Dietert
影响因子：
4.8
作者：
Burk, Raymond F.;Olson, Gary E.;Kurokawa, Suguru
通讯作者：
Kurokawa, Suguru
影响因子：
5.1
作者：
Zhang, Wen;Zhang, Runxiang;Zhang, Naisheng
通讯作者：
Zhang, Naisheng
影响因子：
5.1
作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang
通讯作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang