Hyperbaric Oxygen Treatment Following Mid-Cervical Spinal Cord Injury Preserves Diaphragm Muscle Function.
Hyperbaric Oxygen Treatment Following Mid-Cervical Spinal Cord Injury Preserves Diaphragm Muscle Function.
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DOI：
10.3390/ijms21197219
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发表时间：
2020-09-30
影响因子：
5.6
通讯作者：
Fuller DD
中科院分区：
文献类型：
--
作者：
Smuder AJ;Turner SM;Schuster CM;Morton AB;Hinkley JM;Fuller DD
由于颈脊髓损伤（SCI）导致的对膈肌的氧化损伤促进肌肉萎缩和虚弱。呼吸功能不全是颈脊髓损伤（SCI）患者发病率和死亡率的主要原因，强调了维持膈肌功能策略的必要性。高压氧（HBO）增加溶解到血液中的氧气量，提高氧气输送到骨骼肌和活性氧（ROS）的产生。有人提出，由于HBO治疗增强ROS的产生刺激适应隔膜氧化能力，导致氧化应激和炎症的整体减少。因此，我们测试了这样一个假设，即SCI后急性暴露于HBO治疗会减少对膈肌的氧化损伤，保持肌纤维的大小和收缩力。我们的研究结果表明，在C3/4侧挫伤的结果在膈肌特异性生产力和纤维横截面积，这是与线粒体过氧化氢排放量增加和线粒体呼吸控制率降低显着减少。相比之下，大鼠进行SCI，然后HBO暴露组成的1小时的100%的氧气在3个大气压绝对（ATA）连续10天交付表现出改善的脊髓特异性的力量生产，和衰减的纤维萎缩，线粒体功能障碍和ROS的生产。这些有益的适应性调整，在隔膜有关HBO诱导的抗氧化能力的增加和减少atrogene表达。这些结果表明，高压氧治疗可能是一个有效的后续治疗，以促进呼吸健康的颈椎脊髓损伤。
Oxidative damage to the diaphragm as a result of cervical spinal cord injury (SCI) promotes muscle atrophy and weakness. Respiratory insufficiency is the leading cause of morbidity and mortality in cervical spinal cord injury (SCI) patients, emphasizing the need for strategies to maintain diaphragm function. Hyperbaric oxygen (HBO) increases the amount of oxygen dissolved into the blood, elevating the delivery of oxygen to skeletal muscle and reactive oxygen species (ROS) generation. It is proposed that enhanced ROS production due to HBO treatment stimulates adaptations to diaphragm oxidative capacity, resulting in overall reductions in oxidative stress and inflammation. Therefore, we tested the hypothesis that exposure to HBO therapy acutely following SCI would reduce oxidative damage to the diaphragm muscle, preserving muscle fiber size and contractility. Our results demonstrated that lateral contusion injury at C3/4 results in a significant reduction in diaphragm muscle-specific force production and fiber cross-sectional area, which was associated with augmented mitochondrial hydrogen peroxide emission and a reduced mitochondrial respiratory control ratio. In contrast, rats that underwent SCI followed by HBO exposure consisting of 1 h of 100% oxygen at 3 atmospheres absolute (ATA) delivered for 10 consecutive days demonstrated an improvement in diaphragm-specific force production, and an attenuation of fiber atrophy, mitochondrial dysfunction and ROS production. These beneficial adaptations in the diaphragm were related to HBO-induced increases in antioxidant capacity and a reduction in atrogene expression. These findings suggest that HBO therapy may be an effective adjunctive therapy to promote respiratory health following cervical SCI.
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影响因子：
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作者：
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影响因子：
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影响因子：
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