Targeting the Gut Microbiota and Host Immunity with a Bacilli-Species Probiotic during Antibiotic Exposure in Mice.
Targeting the Gut Microbiota and Host Immunity with a Bacilli-Species Probiotic during Antibiotic Exposure in Mice.
复制标题
小鼠抗生素暴露期间，靶向肠道微生物群和宿主免疫。
DOI：
10.3390/microorganisms10061178
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发表时间：
2022-06-08
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
抗生素治疗是治疗细菌感染所必需的;然而，它也会破坏肠道微生物的平衡和功能，并对宿主产生负面影响。益生菌已被测试为抵消抗生素治疗负面影响的一种手段，但许多益生菌在一起服用时也可能被抗生素破坏。在此，我们旨在测试在抗生素治疗期间提供的含有益生菌混合物的非致病性孢子形成芽孢杆菌物种对小鼠中宿主免疫防御的功效。将小鼠暴露于抗生素并补充或不补充益生菌混合物，并与对照小鼠进行比较。分析粪便和盲肠内容物中的肠道微生物，并测试肠组织中参与维生素A代谢的关键酶、血清淀粉样蛋白A和肠道炎症标志物的表达。益生菌混合物可防止盲肠中革兰氏阴性菌和γ-变形菌的过度生长，这与宿主免疫反应有关。抗生素组之间发生了与维生素A代谢有关的酶的mRNA表达的区域反应，益生菌混合物减轻了肠道炎症标志物。这些数据表明，预防性补充含有芽孢杆菌的益生菌可以防止宿主免疫应答的失调。
Antibiotic therapy is necessary for the treatment of bacterial infections; however, it can also disrupt the balance and function of commensal gut microbes and negatively affect the host. Probiotics have been tested as a means to counteract the negative effects of antibiotic therapy, but many probiotics are also likely destroyed by antibiotics when taken together. Here we aimed to test the efficacy of a non-pathogenic spore-forming Bacillus-species containing a probiotic blend provided during antibiotic therapy on host immune defenses in mice. Mice were exposed to antibiotics and supplemented with or without the probiotic blend and compared to control mice. Fecal and cecal contents were analyzed for gut microbes, and intestinal tissue was tested for the expression of key enzymes involved in vitamin A metabolism, serum amyloid A, and inflammatory markers in the intestine. The probiotic blend protected against antibiotic-induced overgrowth of gram-negative bacteria and gammaproteobacteria in the cecum which correlated with host immune responses. Regional responses in mRNA expression of enzymes involved with vitamin A metabolism occurred between antibiotic groups, and intestinal inflammatory markers were mitigated with the probiotic blend. These data suggest prophylactic supplementation with a spore-forming Bacillus-containing probiotic may protect against antibiotic-induced dysregulation of host immune responses.
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影响因子：
64.5
作者：
Ayres JS
通讯作者：
Ayres JS
DOI：
10.1126/science.abf9232
发表时间：
2021-09-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bang YJ;Hu Z;Li Y;Gattu S;Ruhn KA;Raj P;Herz J;Hooper LV
通讯作者：
Hooper LV
DOI：
10.1177/0148607113486809
发表时间：
2013-11
期刊：
JPEN. Journal of parenteral and enteral nutrition
影响因子：
--
作者：
Cresci G;Nagy LE;Ganapathy V
通讯作者：
Ganapathy V
DOI：
10.3390/antibiotics6040021
发表时间：
2017-10-12
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Blaabjerg S;Artzi DM;Aabenhus R
通讯作者：
Aabenhus R
影响因子：
64.5
作者：
Lee, June-Yong;Hall, Jason A.;Littman, Dan R.
通讯作者：
Littman, Dan R.