Continuous sheath-free separation of drug-treated human fungal pathogen Cryptococcus neoformans by morphology in biocompatible polymer solutions.
Continuous sheath-free separation of drug-treated human fungal pathogen Cryptococcus neoformans by morphology in biocompatible polymer solutions.
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DOI：
10.1002/elps.201700428
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发表时间：
2018-09
期刊：
影响因子：
2.9
通讯作者：
Xuan X
中科院分区：
文献类型：
--
作者：
Li D;Zielinski J;Kozubowski L;Xuan X
由新生隐球菌引起的隐球菌性脑膜炎是引起中枢神经系统真菌感染的主要原因。目前对隐球菌感染的抗真菌治疗不充分，部分原因是出现了耐药性。最近的研究表明，唑类药物氟康唑的治疗改变了新生葡萄球菌的形态，形成了由三个或三个以上相连的细胞/芽组成的扩大的多聚体。为了分析这些多聚体细胞是否是新生葡萄球菌获得耐药性的先决条件，能够将它们从正常细胞中分离出来的工具至关重要。我们将我们最近展示的无鞘弹性惯性粒子分离技术扩展到分级药物处理的新生隐形杆菌细胞，通过在生物相容的聚合物溶液中进行形态观察。根据效率、纯度和富集率这三个无量纲指标，对多聚体和普通电池的分离性能进行了评估。研究了流速、聚合物浓度和微通道高度对细胞分离的影响。
Cryptococcal meningitis caused by Cryptococcus neoformans is the leading cause of fungal central nervous system infections. Current antifungal treatments for cryptococcal infections are inadequate partly due to the occurrence of drug resistance. Recent studies indicate that the treatment of the azole drug fluconazole changes the morphology of C. neoformans to form enlarged “multimeras” that consist of three or more connected cells/buds. To analyze if these multimeric cells are a prerequisite for C. neoformans to acquire drug resistance, a tool capable of separating them from normal cells is critical. We extend our recently demonstrated sheath-free elasto-inertial particle separation technique to fractionate drug-treated C. neoformans cells by morphology in biocompatible polymer solutions. The separation performance is evaluated for both multimeric and normal cells in terms of three dimensionless metrics: efficiency, purity and enrichment ratio. The effects of flow rate, polymer concentration and microchannel height on cell separation are studied.
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