Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus.
Structural, antigenic, and evolutionary characterizations of the envelope protein of newly emerging Duck Tembusu Virus.
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DOI：
10.1371/journal.pone.0071319
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun H
中科院分区：
文献类型：
--
作者：
Yu K;Sheng ZZ;Huang B;Ma X;Li Y;Yuan X;Qin Z;Wang D;Chakravarty S;Li F;Song M;Sun H
自2010年4月在中国东部首次报告鸭感染一种以前未知的黄病毒病例以来，这种暂时被称为鸭坦布苏病毒（DTMUV）的病毒在中国家鸭中广泛传播，给家禽业造成了重大经济损失。在这项研究中，我们详细检查了2010-2012年期间6株DTMUV分离株的包膜(E)蛋白的结构、抗原性和进化特性，每株分离株均来自记录疾病暴发的不同地理位置的单个农场。结构分析表明，DTMUV及其密切相关的黄病毒（日本脑炎病毒）的E蛋白具有保守的预测功能域和基序序列。在6株DTMUV菌株中，仅在E蛋白3个不同结构域的13个氨基酸位置观察到突变。有趣的是，在血清中和试验中，这些基因多态性没有导致病毒中和特性的可检测变化。此外，E蛋白核苷酸序列的系统发育分析表明，病毒进化成两种不同的基因型，称为DTMUV。I和DTMUV。II，其中II成为优势基因型。本文描述的新发现将有助于深入了解这种新病原体的抗原性和进化，并为尚未开发出有效疫苗的E蛋白的进一步功能研究提供指导。
Since the first reported cases of ducks infected with a previously unknown flavivirus in eastern China in April 2010, the virus, provisionally designated Duck Tembusu Virus (DTMUV), has spread widely in domestic ducks in China and caused significant economic losses to poultry industry. In this study, we examined in detail structural, antigenic, and evolutionary properties of envelope (E) proteins of six DTMUV isolates spanning 2010–2012, each being isolated from individual farms with different geographical locations where disease outbreaks were documented. Structural analysis showed that E proteins of DTMUV and its closely related flavivirus (Japanese Encephalitis Virus) shared a conserved array of predicted functional domains and motifs. Among the six DTMUV strains, mutations were observed only at thirteen amino acid positions across three separate domains of the E protein. Interestingly, these genetic polymorphisms resulted in no detectable change in viral neutralization properties as demonstrated in a serum neutralization assay. Furthermore, phylogenetic analysis of the nucleotide sequences of the E proteins showed that viruses evolved into two distinct genotypes, termed as DTMUV.I and DTMUV.II, with II emerging as the dominant genotype. New findings described here shall give insights into the antigenicity and evolution of this new pathogen and provide guidance for further functional studies of the E protein for which no effective vaccine has yet been developed.
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新出现的中国鸭落蛋综合症黄病毒的基因组和抗原特征：与坦布苏病毒和实兆远病毒的基因组比较
影响因子：
3.8
作者：
Liu, Peipei;Lu, Hao;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
4.8
作者：
Davis, Carl W.;Mattei, Lisa M.;Pierson, Theodore C.
通讯作者：
Pierson, Theodore C.
影响因子：
64.8
作者：
Nybakken GE;Oliphant T;Johnson S;Burke S;Diamond MS;Fremont DH
通讯作者：
Fremont DH
DOI：
10.1083/jcb.200806081
发表时间：
2008-10-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fritz R;Stiasny K;Heinz FX
通讯作者：
Heinz FX
影响因子：
11.8
作者：
Cao Z;Zhang C;Liu Y;Liu Y;Ye W;Han J;Ma G;Zhang D;Xu F;Gao X;Tang Y;Shi S;Wan C;Zhang C;He B;Yang M;Lu X;Huang Y;Diao Y;Ma X;Zhang D
通讯作者：
Zhang D