Defense against phytopathogens relies on efficient antimicrobial protein secretion mediated by the microtubule-binding protein TGNap1.
Defense against phytopathogens relies on efficient antimicrobial protein secretion mediated by the microtubule-binding protein TGNap1.
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DOI：
10.1038/s41467-023-41807-4
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发表时间：
2023-10-11
影响因子：
16.6
通讯作者：
Brandizzi, Federica
中科院分区：
文献类型：
--
作者：
Bhandari, Deepak D.;Ko, Dae Kwan;Kim, Sang-Jin;Nomura, Kinya;He, Sheng Yang;Brandizzi, Federica
植物免疫依赖于抗菌蛋白的分泌，而抗菌蛋白的分泌机制尚不清楚。跨高尔基体网络(TGN)是细胞内和细胞外运输途径的枢纽，细胞骨架是分泌抗菌蛋白所必需的，它们正在成为抑制植物免疫的病原体靶标。在这项工作中，我们证明了TGNap1-2，一个与TGN相关的微管结合蛋白，拟南芥TGNap1的功能缺失突变体，对丁香假单胞菌(Pst DC3000)敏感。PST DC3000感染的Tgnap1-2能够动员防御途径，积累水杨酸(SA)，并表达抗菌蛋白。Tgnap1-2的敏感性是由于未能通过部分依赖MT但独立于SA的途径将抗菌蛋白有效地运输到质外体，并与病原体拮抗MIN7(一种与TGN相关的ARF-gef蛋白)相加。因此，我们的数据表明，植物免疫依赖于TGNap1来分泌抗菌蛋白，TGNap1是一个关键的免疫元件，在SA非依赖性病原体反应中将分泌和细胞骨架联系在一起。明确植物对病原菌的防御机制在细胞生物学和作物产量方面具有重要意义。TGNap1是一种TGN和微管结合蛋白，是防御和有效的抗微生物蛋白分泌所必需的，连接分泌和细胞骨架。
Plant immunity depends on the secretion of antimicrobial proteins, which occurs through yet-largely unknown mechanisms. The trans-Golgi network (TGN), a hub for intracellular and extracellular trafficking pathways, and the cytoskeleton, which is required for antimicrobial protein secretion, are emerging as pathogen targets to dampen plant immunity. In this work, we demonstrate that tgnap1-2, a loss-of-function mutant of Arabidopsis TGNap1, a TGN-associated and microtubule (MT)-binding protein, is susceptible to Pseudomonas syringae (Pst DC3000). Pst DC3000 infected tgnap1-2 is capable of mobilizing defense pathways, accumulating salicylic acid (SA), and expressing antimicrobial proteins. The susceptibility of tgnap1-2 is due to a failure to efficiently transport antimicrobial proteins to the apoplast in a partially MT-dependent pathway but independent from SA and is additive to the pathogen-antagonizing MIN7, a TGN-associated ARF-GEF protein. Therefore, our data demonstrate that plant immunity relies on TGNap1 for secretion of antimicrobial proteins, and that TGNap1 is a key immunity element that functionally links secretion and cytoskeleton in SA-independent pathogen responses. Defining plant defense machinery against pathogens is significant in cell biology and crop yield. TGNap1, a TGN and microtubule-binding protein, is required for defense and efficient anti-microbial protein secretion, linking secretion and cytoskeleton.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
16.6
作者：
Bhandari, Deepak D.;Lapin, Dmitry;Parker, Jane E.
通讯作者：
Parker, Jane E.
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
7.2
作者：
Hardham, Adrienne R.
通讯作者：
Hardham, Adrienne R.