Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables.
Identification of exosome-like nanoparticle-derived microRNAs from 11 edible fruits and vegetables.
复制标题
鉴定来自 11 种可食用水果和蔬菜的外泌体样纳米颗粒衍生的 microRNA
作者：
Xiao J;Feng S;Wang X;Long K;Luo Y;Wang Y;Ma J;Tang Q;Jin L;Li X;Li M
可食用植物来源的类外切体纳米颗粒(EPDELN)是一种新型的天然存在的植物超微结构，其结构与外切体相似。许多EPDELN具有抗炎特性。MicroRNAs(MiRNAs)在动物和植物的生理和病理过程中发挥着重要的作用。虽然miRNAs可以选择性地包裹在胞外小泡中，但对其在EPDELN中的表达和功能知之甚少。在本研究中，我们从11种食用水果和蔬菜中分离出纳米囊泡，并对相应的EPDELN小RNA文库进行了Illumina测序。我们从11个EPDELN样本中鉴定出总共418个miRNAs--每个物种32到127个。靶点预测和功能分析表明，miRNAs的高表达与炎症反应和癌症相关途径密切相关。根据EPDELN的分布，418个miRNAs可分为三类：26个“频繁”miRNAs(Fms)，39个“中度存在”miRNAs(MPM)和353个“稀有”miRNAs(RMS)。与RMS相比，FMS的miRNA种类较少，但累积表达水平显着高于RMS。综上所述，我们的体外结果表明，EPDELN中的miRNAs具有调节人类mRNA的潜力。
Edible plant-derived exosome-like nanoparticles (EPDELNs) are novel naturally occurring plant ultrastructures that are structurally similar to exosomes. Many EPDELNs have anti-inflammatory properties. MicroRNAs (miRNAs) play a critical role in mediating physiological and pathological processes in animals and plants. Although miRNAs can be selectively encapsulated in extracellular vesicles, little is known about their expression and function in EPDELNs. In this study, we isolated nanovesicles from 11 edible fruits and vegetables and subjected the corresponding EPDELN small RNA libraries to Illumina sequencing. We identified a total of 418 miRNAs—32 to 127 per species—from the 11 EPDELN samples. Target prediction and functional analyses revealed that highly expressed miRNAs were closely associated with the inflammatory response and cancer-related pathways. The 418 miRNAs could be divided into three classes according to their EPDELN distributions: 26 “frequent” miRNAs (FMs), 39 “moderately present” miRNAs (MPMs), and 353 “rare” miRNAs (RMs). FMs were represented by fewer miRNA species than RMs but had a significantly higher cumulative expression level. Taken together, our in vitro results indicate that miRNAs in EPDELNs have the potential to regulate human mRNA.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
3.5
作者：
Fairfax BP;Vannberg FO;Radhakrishnan J;Hakonarson H;Keating BJ;Hill AV;Knight JC
通讯作者：
Knight JC
影响因子：
3
作者：
Lu, Yuzhu;Feng, Zhen;Liang, Jiansheng
通讯作者：
Liang, Jiansheng
影响因子：
4.2
作者：
Ding, Dong;Zhang, Lifang;Zheng, Yonglian
通讯作者：
Zheng, Yonglian
影响因子：
12.4
作者：
Ju, Songwen;Mu, Jingyao;Zhang, Huang-Ge
通讯作者：
Zhang, Huang-Ge