Direct and quantitative analysis of underivatized acylcarnitines in serum and whole blood using paper spray mass spectrometry.
Direct and quantitative analysis of underivatized acylcarnitines in serum and whole blood using paper spray mass spectrometry.
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DOI：
10.1007/s00216-012-6211-4
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发表时间：
2012-09
影响因子：
4.3
通讯作者：
Ouyang, Zheng
中科院分区：
文献类型：
--
作者：
Yang, Qian;Manicke, Nicholas E.;Wang, He;Petucci, Christopher;Cooks, R. Graham;Ouyang, Zheng
本文建立了一种简便的纸喷雾质谱法快速测定血清和全血中酰基肉毒碱的方法。干血清和全血中含有不同浓度的10种酰基肉毒碱的混合物，直接从纸上分析斑点，无需任何样品预处理，分离或衍生。发现喷雾溶剂的组成是一个关键因素：对于血清样品，甲醇/水/甲酸（80：20：0.1）的喷雾溶剂提供了最佳的信号强度，而对于含有更多基质组分的血液样品，乙腈/水（90：10）是更合适的喷雾溶剂。对于所使用的纸张类型和尺寸，0.5 μL样本可为血清和全血样本提供最佳信号。对于定量分析，从血清和血液中获得的定量限远低于新生儿筛查中诊断脂肪酸氧化障碍的临床验证临界值。在100 nM至5 μM的浓度范围内，C2酰基肉毒碱具有线性（R2>0.95）和重现性（RSD ~ 10%），而对于其他酰基肉毒碱，这些值为10至500 nM。酰基肉毒碱的配置文件提供了一个有效的事实证明，纸喷雾质谱法是一个适当的，简单的，快速的方法，具有高灵敏度和高重现性适用于新生儿筛查试验。
A simple protocol for rapid quantitation of acylcarnitines in serum and whole blood has been developed using paper spray mass spectrometry. Dried serum and whole blood containing a mixture of ten acylcarnitines at various concentrations were analyzed as spots from paper directly without any sample pretreatment, separation, or derivatization. The composition of the spray solvent was found to be a critical factor: for serum samples, spray solvent of methanol/water/formic acid (80:20:0.1) gave the best signal intensity while for blood samples which contain more matrix components, acetonitrile/water (90:10) was a much more suitable spray solvent. For the paper type and size used, 0.5 μL of sample provided an optimal signal for both serum and whole blood samples. For quantitative profiling, the limits of quantitation obtained from both serum and blood were much lower than the clinically validated cutoff values for diagnosis of fatty acid oxidation disorders in newborn screening. Linearity (R2>0.95) and reproducibility (RSD ~10 %) were achieved in the concentration ranges from 100 nM to 5 μM for the C2 acylcarnitine, and for other acylcarnitines, these values were from 10 to 500 nM. Acylcarnitine profiles offer an effective demonstration of the fact that paper spray mass spectrometry is an appropriate, simple, rapid method with high sensitivity and high reproducibility applicable to newborn screening tests.
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影响因子：
7.4
作者：
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通讯作者：
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