Human monocyte-derived soluble product(s) has an accessory function in the generation of histamine- and concanavalin A-induced suppressor T cells.
Human monocyte-derived soluble product(s) has an accessory function in the generation of histamine- and concanavalin A-induced suppressor T cells.
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人单核细胞来源的可溶性产物在组胺和刀豆球蛋白 A 诱导的抑制性 T 细胞的生成中具有辅助功能。
DOI：
10.1172/jci110629
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Rocklin,RE
中科院分区：
文献类型：
--
作者：
Beer,DJ;Dinarello,CA;Rosenwasser,LJ;Rocklin,RE
我们分析了产生组胺和豆豆蛋白A （Con A）诱导的抑制性T细胞所需的细胞相互作用，方法是采用共培养实验和分离人血单个核细胞（PBMC）的技术。PBMC在组胺（0.1 mM-1 mM）或Con A（20微克/ml）存在下培养24小时，丝裂霉素处理后，随后与自体丝裂原刺激的单核细胞联合，显著抑制了随后的成母反应。PBMC在尼龙羊毛柱上分离后，失去了贴壁细胞，富集了T细胞（NWNA-T），无法产生抑制活性。然而，NWNA-T细胞的抑制细胞功能是通过添加自体单核细胞来重建的。在组胺和ConA抑制系统中，通过将NWNA-T群体悬浮在由热杀死的白色葡萄球菌刺激的同种异体单核细胞衍生的上清液中，可以增强激活过程中对单核细胞的需求。这些粗上清含有白细胞热原（LP）和淋巴细胞活化因子（LAF）。使用凝胶过滤、免疫吸附和等电聚焦对粗上清进行顺序纯化和分离表明，只有含有LP和LAF的部分能够重建NWNA-T细胞组胺和Con a诱导的抑制活性。因此，这些研究表明，来自活化单核细胞的上清在抑制细胞产生中的辅助作用可能是由LP/LAF介导的。进一步的研究正在探索可溶性因子刺激抑制性T细胞的机制。
We have analyzed the cellular interactions required for the generation of histamine- and concanavalin A (Con A)-induced suppressor T cells by employing a co-culture assay and techniques for fractionation of human blood mononuclear cells (PBMC). PBMC cultured in the presence of histamine (0.1 mM-1 mM) or Con A (20 micrograms/ml) for 24 h, mitomycin treated and subsequently combined with autologous mitogen-stimulated mononuclear cells, significantly suppressed a subsequent blastogenic response. PBMC fractionated over nylon wool columns and depleted of adherent cells and enriched for T cells (NWNA-T) were unable to generate suppressor activity. However, suppressor cell function by NWNA-T cells was reconstituted by the addition of autologous monocytes. In both the histamine and ConA suppressor systems, the requirement for monocytes in the activation process was enhanced by suspending the NWNA-T population in supernatants derived from allogeneic monocytes stimulated with heat-killed Staphylococcus albus. These crude supernatants contained leukocytic pyrogen (LP) and lymphocyte activating factor (LAF). Sequential purification and separation of the crude supernatants using gel-filtration, immunoadsorption, and isoelectric focusing demonstrated that only those fractions containing LP and LAF were capable to reconstituting NWNA-T cell histamine and Con A-induced suppressor activity. Thus, these studies suggest that the accessory role of supernatants derived from activated monocytes in the generation of suppressor cells may be mediated by LP/LAF. Further studies are in progress to explore the mechanism by which soluble factors stimulate suppressor T cells.
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影响因子：
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