Lung infection of avian pathogenic Escherichia coli co-upregulates the expression of cSP-A and cLL in chickens.
Lung infection of avian pathogenic Escherichia coli co-upregulates the expression of cSP-A and cLL in chickens.
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禽致病性大肠杆菌的肺部感染共同上调鸡中 cSP-A 和 cLL 的表达。
DOI：
10.1016/j.rvsc.2022.07.023
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发表时间：
2022-08
影响因子：
2.4
通讯作者：
Hongmei Liu
中科院分区：
文献类型：
--
作者：
Hanwen Wu;Haifeng Xiong;Xueting Huang;Qian Zhou;Dongmei Hu;Kezong Qi;Hongmei Liu
宿主先天防御与病原体在肺部的相互作用一直是人们关注的话题。呼吸道是禽致病性大肠杆菌（APEC）的常见进入途径。鸡表面活性蛋白A（cSP-A）和鸡肺凝集素（cLL）可以与各种微生物的碳水化合物部分结合。尽管在鸡中检测到它们，但它们在先天免疫应答中的作用在很大程度上是未知的。本研究旨在研究禽流感病毒感染对鸡呼吸系统cSP-A和cLL表达水平的影响。用5日龄无特异性病原体的雏鸡经气管内感染APEC，建立体内肺定植模型。分别于感染后12 h（hpi）和感染后1-3 d（dpi）处死鸡，检测各项指标。定量逆转录-聚合酶链反应（RT-PCR）和荧光多重免疫组织化学染色结果显示，感染后2 d，肺和气管中cSP-A和cLL的mRNA和蛋白表达水平显著上调;转录组RNA测序分析表明，感染后2 d，与对照鸡相比，约有810个基因表达差异，但只有少数基因的表达差异有统计学意义。cLL和cSP-A是参与先天免疫的显著上调的基因。提示cSP-A和cLL可能在肺固有宿主防御APEC感染的早期阶段起重要作用。
The host innate defense-pathogen interaction in the lung has always been a topic of concern. The respiratory tract is a common entry route for Avian pathogenic Escherichia coli (APEC). Chicken surfactant protein A (cSP-A) and chicken lung lectin (cLL) can bind to the carbohydrate moieties of various microorganisms. Despite their detection in chickens, their role in the innate immune response is largely unknown. This study aimed to examine whether the expression levels of cSP-A and cLL in the chicken respiratory system were affected by APEC infection. A lung colonization model was established in vivo using 5-day-old specific-pathogen-free chickens infected intratracheally with APEC. The chickens were euthanized 12 h post-infection (hpi) and 1-3 days post-infection (dpi) to detect various indicators. The results of quantitative reverse transcription-polymerase chain reaction and fluorescence multiplex immunohistochemical staining showed that the mRNA and protein expression levels of cSP-A and cLL in the lung and trachea were significantly co-upregulated at 2dpi.Transcriptome RNA-sequencing analysis indicated that the inoculation with APEC AE17 at 2 dpi resulted in differential gene expression of approximately 810 genes compared with control birds, but only a few genes were expressed with astatistically significant ≧2-fold difference. cLL and cSP-A were among the significantly upregulated genes involved in innate immunity. These findings indicated that cSP-A and cLL might play an important role in lung innate host defense against APEC infection at the early stage.
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影响因子：
3.7
作者：
Sotiriadis G;Dodagatta-Marri E;Kouser L;Alhamlan FS;Kishore U;Karteris E
通讯作者：
Karteris E
影响因子：
3.3
作者：
Hogenkamp, Astrid;Isohadouten, Najiha;Haagsman, Henk P.
通讯作者：
Haagsman, Henk P.
影响因子：
3.3
作者：
Reemers, Sylvia S.;Veldhuizen, Edwin J. A.;Vervelde, Lonneke
通讯作者：
Vervelde, Lonneke
影响因子：
6.7
作者：
Mutua MP;Muya S;Gicheru MM
通讯作者：
Gicheru MM
禽致病性大肠埃希菌（APEC）感染鸭免疫相关基因的表达
影响因子：
5.2
作者：
Li R;Li N;Zhang J;Wang Y;Liu J;Cai Y;Chai T;Wei L
通讯作者：
Wei L