Development of 68Ga-labeled tin colloids for evaluating phagocytic function of Kupffer cells using preclinical PET imaging
Development of 68Ga-labeled tin colloids for evaluating phagocytic function of Kupffer cells using preclinical PET imaging
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开发 68Ga 标记的锡胶体，用于使用临床前 PET 成像评估 Kupffer 细胞的吞噬功能
DOI：
10.1007/s12149-020-01505-3
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发表时间：
2020
影响因子：
2.6
通讯作者：
Jinzaki Masahiro
中科院分区：
文献类型：
--
作者：
Matsusaka Yohji;Nakahara Tadaki;Takahashi Kazuhiro;Iwabuchi Yu;Nakamura Shoki;Jinzaki Masahiro
本研究旨在探讨68 Ga标记锡胶体的最佳制备条件以及68 Ga标记锡胶体正电子发射断层扫描（PET）显像评价枯否细胞（KCs）吞噬功能的可行性OmL）加入到68Ga溶液（I. OmL）中，随后用乙酸钠（1M，0.2mL）调节pH。对不同的标记时间进行了测试，以找到最佳的标记时间。通过三层膜过滤器（孔径为200、3000和5000 nm）过滤溶液，测量胶体尺寸，并使用γ计数器测量整个滤液和过滤器中的放射性。通过在环境条件下孵育长达60分钟后的尺寸测量来评价胶体的体外稳定性。健康大鼠静脉注射68Ga-锡胶体溶液（4MBq）后进行PET扫描30min。生成肝脏、脾脏和血池的时间-活性曲线。最后，肝摄取之前和之后的KC耗尽和非酒精性脂肪性肝炎（NASH）大鼠models.ResultsColloid的大小随着标记时间的增加而增加。pH调节后，胶体大小几乎保持不变。最佳标记时间为30 min。健康大鼠的PET成像显示，肝脏对68 Ga-锡胶体的摄取随胶体大小的增加而增加。在KC耗尽的大鼠中，肝摄取显著降低（n = 4，p <0.01）。NASH模型大鼠肝脏对68Ga-锡胶体的摄取明显降低（n = 5，p <0.01）。结论用放射性标记法制备的68Ga-锡胶体可用于体内PET显像评价KC的吞噬功能。
ObjectiveThis study aimed to investigate the optimal conditions for producing68Ga-labeled tin colloid and the feasibility of68Ga-tin colloid positron emission tomography (PET) for visualization and evaluation of the phagocytic function of Kupffer cells (KCs) in vivo.Methods68Ga-tin colloid was prepared by adding tin solution (1 mM, 0.2 mL) to68Ga solution (1.0 mL), followed by pH adjustment with sodium acetate (1 M, 0.2 mL). Various labeling times were tested to find the optimal one. Colloid size was measured by filtering the solution through three-ply membrane filters (with pore sizes of 200, 3000, and 5000 nm), and radioactivity was measured in the whole filtrate and the filters using a gamma counter. The in vitro stability of the colloid was evaluated by the size measurement after incubation under ambient conditions for up to 60 min. PET scanning was performed for 30 min after intravenous administration of68Ga-tin colloid solution (4 MBq) to healthy rats. Time-activity-curves for the liver, spleen, and blood pool were generated. Finally, liver uptake was compared before and after the establishment of KC-depletion and non-alcoholic steatohepatitis (NASH) rat models.ResultsColloid size increased with increasing labeling time. After pH adjustment, the colloid sizes remained nearly unchanged. The optimal labeling time was determined as 30 min. PET imaging of healthy rats revealed that liver uptake of the68Ga-tin colloid increased with increasing colloid size. In KC-depleted rats, liver uptake significantly decreased (n= 4,p< 0.01). NASH model rats showed significantly decreased uptake of68Ga-tin colloid in the livers (n= 5,p< 0.01).Conclusions68Ga-tin colloid, prepared by a simple radiolabeling method, enabled in vivo PET imaging to evaluate the phagocytic function of KCs.
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影响因子：
5.8
作者：
Dixon LJ;Barnes M;Tang H;Pritchard MT;Nagy LE
通讯作者：
Nagy LE
非酒精性脂肪性肝炎的胶体闪烁扫描：新发疾病的常规诊断方法
影响因子：
1.5
作者：
D. Duman;F. Dede;H. Akin;F. Şen;H. Turoğlu;Ç. Çelikel;N. Tözün
通讯作者：
N. Tözün
比较粒径对放射性胶体在患者体内分布影响的交叉研究
影响因子：
10.6
作者：
R. Kloiber;B. Damtew;L. Rosenthall
通讯作者：
L. Rosenthall
影响因子：
24.5
作者：
Hoffmann, F.;Sass, G.;Vollmar, A. M.
通讯作者：
Vollmar, A. M.
放射化学原理
DOI：
--
发表时间：
1971
期刊：
--
影响因子：
--
作者：
H. Mckay
通讯作者：
H. Mckay