Expression of selected Ginkgo biloba heat shock protein genes after cold treatment could be induced by other abiotic stress.
Expression of selected Ginkgo biloba heat shock protein genes after cold treatment could be induced by other abiotic stress.
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DOI：
10.3390/ijms13055768
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发表时间：
2012
影响因子：
5.6
通讯作者：
Yuan H
中科院分区：
文献类型：
--
作者：
Cao F;Cheng H;Cheng S;Li L;Xu F;Yu W;Yuan H
热激蛋白（HSPs）在植物中具有多种逆境保护作用。本研究从低温胁迫下银杏叶片的抑制性消减杂交（SSH）cDNA文库中分离到3个HSP基因。基于分子量，这三个基因被命名为GbHSP 16.8、GbHSP 17和GbHSP 70。结果表明，这3个基因分别编码149个、152个和657个氨基酸的多肽链，其分子量分别为16.67、17.39和71.81 kDa。这三个基因在不同器官或发育阶段表现出不同的表达模式。GbHSP16.8和GbHSP 70在叶片中表达量较高，在雌蕊中表达量较低; GbHSP 17在雄蕊中表达量较高，在果实中表达量较低。结果表明，GbHSP 16.8和GbHSP 70在银杏叶片发育和光合作用中起重要作用，GbHSP 17在花粉成熟过程中起积极作用。低温胁迫下3种GbHSPs均表达上调，极端高温胁迫仅引起GbHSP 70表达上调，UV-B处理引起GbHSP 16. 8和GbHSP 17表达上调，创伤处理引起GbHSP 16. 8和GbHSP 70表达上调，脱落酸（阿坝）处理主要引起GbHSP 70表达上调。
Heat shock proteins (HSPs) play various stress-protective roles in plants. In this study, three HSP genes were isolated from a suppression subtractive hybridization (SSH) cDNA library of Ginkgo biloba leaves treated with cold stress. Based on the molecular weight, the three genes were designated GbHSP16.8, GbHSP17 and GbHSP70. The full length of the three genes were predicted to encode three polypeptide chains containing 149 amino acids (Aa), 152 Aa, and 657 Aa, and their corresponding molecular weights were predicted as follows: 16.67 kDa, 17.39 kDa, and 71.81 kDa respectively. The three genes exhibited distinctive expression patterns in different organs or development stages. GbHSP16.8 and GbHSP70 showed high expression levels in leaves and a low level in gynoecia, GbHSP17 showed a higher transcription in stamens and lower level in fruit. This result indicates that GbHSP16.8 and GbHSP70 may play important roles in Ginkgo leaf development and photosynthesis, and GbHSP17 may play a positive role in pollen maturation. All three GbHSPs were up-regulated under cold stress, whereas extreme heat stress only caused up-regulation of GbHSP70, UV-B treatment resulted in up-regulation of GbHSP16.8 and GbHSP17, wounding treatment resulted in up-regulation of GbHSP16.8 and GbHSP70, and abscisic acid (ABA) treatment caused up-regulation of GbHSP70 primarily.
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影响因子：
4.8
作者：
Lewis, J;Devin, A;Liu, ZG
通讯作者：
Liu, ZG
影响因子：
4.2
作者：
Leonardi, R;Villari, L;Travali, S
通讯作者：
Travali, S
影响因子：
8
作者：
Mayer, MP;Bukau, B
通讯作者：
Bukau, B
影响因子：
4.3
作者：
Löw, D;Brändle, K;Forreiter, C
通讯作者：
Forreiter, C
影响因子：
4.3
作者：
Gabriel Bondino, Hernan;Marta Valle, Estela;ten Have, Arjen
通讯作者：
ten Have, Arjen