Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.
Subcellular Transcriptomics and Proteomics: A Comparative Methods Review.
复制标题
DOI：
10.1016/j.mcpro.2021.100186
复制
发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Lilley KS
中科院分区：
文献类型：
--
作者：
Christopher JA;Geladaki A;Dawson CS;Vennard OL;Lilley KS
细胞的内部环境是分子拥挤的，这就需要通过亚细胞分区来组织空间。这些隔室为分子执行其生物功能提供了特定的条件，如协调细胞周期、细胞生存和生长。这种划分也不是一成不变的，分子在这些亚细胞邻里之间流动，以执行它们的功能。例如，一些生物分子是多功能的，需要一个具有不同条件或相互作用的伙伴的环境，而另一些生物分子则需要通过交通来输出这些分子。蛋白质或RNA物种的异常定位与许多病理情况有关，如神经、癌症和肺部疾病。转录组学和蛋白质组学中的差异表达研究相对常见，但大多数研究忽略了亚细胞信息的重要性。此外，由于翻译过程中和翻译后发生的生化过程，亚细胞转录组学和蛋白质组学数据并不总是共存，这突出了这些领域的互补性。在这篇综述中，我们讨论并直接比较空间蛋白质组学和转录组学中的当前方法，包括基于测序和基于成像的策略，以使读者对当前可用的工具有一个概述。我们还讨论了这些策略目前的局限性以及空间组学领域的未来发展。蛋白质和RNA的亚细胞信息提供了对分子功能的洞察。这篇综述讨论了可用于测量亚细胞信息的策略。方法的杂交显示了探索细胞器组成的前景。进展有助于理解细胞的组织和动力学。细胞内部是分子拥挤的。它在细胞器内的区隔作用使生化过程得以调节，并允许蛋白质和RNA的多功能。因此，亚细胞信息可以深入了解这些生物分子的功能。已经建立了多种技术来测量这种信息，并且吞吐量和灵敏度都在不断增加。本综述涵盖了这些技术，并展示了它们的应用为细胞生物学提供了有价值的见解，例如帮助我们理解单细胞异质性和翻译后修饰。
The internal environment of cells is molecularly crowded, which requires spatial organization via subcellular compartmentalization. These compartments harbor specific conditions for molecules to perform their biological functions, such as coordination of the cell cycle, cell survival, and growth. This compartmentalization is also not static, with molecules trafficking between these subcellular neighborhoods to carry out their functions. For example, some biomolecules are multifunctional, requiring an environment with differing conditions or interacting partners, and others traffic to export such molecules. Aberrant localization of proteins or RNA species has been linked to many pathological conditions, such as neurological, cancer, and pulmonary diseases. Differential expression studies in transcriptomics and proteomics are relatively common, but the majority have overlooked the importance of subcellular information. In addition, subcellular transcriptomics and proteomics data do not always colocate because of the biochemical processes that occur during and after translation, highlighting the complementary nature of these fields. In this review, we discuss and directly compare the current methods in spatial proteomics and transcriptomics, which include sequencing- and imaging-based strategies, to give the reader an overview of the current tools available. We also discuss current limitations of these strategies as well as future developments in the field of spatial -omics. Subcellular information of protein and RNA give insights into molecular function. This review discusses strategies available to measure subcellular information. Hybridization of methods shows promise for exploring the composition of organelles. Advances are aiding understanding of the organisation and dynamics of cells. The interior of the cell is molecularly crowded. Its compartmentalization within organelles enables the regulation of biochemical processes and allows multifunctionality of proteins and RNAs. Subcellular information can thus give insights into the function of these biomolecules. Multiple techniques to measure such information have been established, with ever-increasing throughput and sensitivity. These techniques are covered in this review, and demonstrating their application is providing valuable insights into cellular biology, such as aiding our understanding of single-cell heterogeneity and posttranslational modifications.
登录
查看更多内容
影响因子：
11.1
作者：
Birnbaum KD
通讯作者：
Birnbaum KD
影响因子：
64.5
作者：
Bhatt DM;Pandya-Jones A;Tong AJ;Barozzi I;Lissner MM;Natoli G;Black DL;Smale ST
通讯作者：
Smale ST
影响因子：
22.7
作者：
Ali, H. Raza;Jackson, Hartland W.;Bodenmiller, Bernd
通讯作者：
Bodenmiller, Bernd
影响因子：
12.3
作者：
Abdelmoez MN;Iida K;Oguchi Y;Nishikii H;Yokokawa R;Kotera H;Uemura S;Santiago JG;Shintaku H
通讯作者：
Shintaku H
影响因子：
9.2
作者：
Andersen, JS;Lyon, CE;Lamond, AI
通讯作者：
Lamond, AI