Power in Numbers: Single-Cell RNA-Seq Strategies to Dissect Complex Tissues.
Power in Numbers: Single-Cell RNA-Seq Strategies to Dissect Complex Tissues.
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DOI：
10.1146/annurev-genet-120417-031247
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发表时间：
2018-11-23
影响因子：
11.1
通讯作者：
Birnbaum KD
中科院分区：
文献类型：
--
作者：
Birnbaum KD
最近单细胞rna-seq规模的增加和成本的下降现在允许重复细胞采样，这增加了检测稀有细胞状态、重建发育轨迹和用细胞变异等新术语测量表型的能力。自从实时荧光显微镜取得突破性进展以来，解剖学和发育动力学的表征还没有取得同等的突破。新的解决方案对遗传学来说是一个福音，因为对表型的新描述提供了完善基因功能和剖析多效性的机会。此外，最近高通量基因扰动与单细胞RNA-SEQ的配对使基因筛查的规模达到了前所未有的水平。
Recent increases in scale and decline in cost of single-cell RNA-seq now permit a repetition of cell sampling that increases the power to detect rare cell states, reconstruct developmental trajectories, and measure phenotype in new terms like cellular variance. The characterization of anatomy and developmental dynamics have not had an equivalent breakthrough since groundbreaking advances in live fluorescent microscopy. The new resolution is a boon to genetics, as the novel description of phenotype offers the opportunity to refine gene function and dissect pleiotropy. In addition, the recent pairing of high-throughput genetic perturbation with single-cell RNA-seq has made practical a scale of genetic screening not previously possible.
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影响因子：
64.5
作者：
Enge M;Arda HE;Mignardi M;Beausang J;Bottino R;Kim SK;Quake SR
通讯作者：
Quake SR
影响因子：
12.3
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Efroni I;Ip PL;Nawy T;Mello A;Birnbaum KD
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Birnbaum KD
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23.9
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Guo J;Grow EJ;Yi C;Mlcochova H;Maher GJ;Lindskog C;Murphy PJ;Wike CL;Carrell DT;Goriely A;Hotaling JM;Cairns BR
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Cairns BR
影响因子：
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Angermueller C;Clark SJ;Lee HJ;Macaulay IC;Teng MJ;Hu TX;Krueger F;Smallwood S;Ponting CP;Voet T;Kelsey G;Stegle O;Reik W
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Reik W
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64.5
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Dixit, Atray;Pamas, Oren;Li, Biyu;Chen, Jenny;Fulco, Charles P.;Jerby-Amon, Livnat;Marjanovic, Nemanja D.;Dionne, Danielle;Burks, Tyler;Raychowdhury, Raktima;Adamson, Britt;Norman, Thomas M.;Lander, Eric S.;Weissman, Jonathan S.;Friedman, Nir;Regev, Aviv
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Regev, Aviv