Determination of meropenem in bacterial media by LC-MS/MS.
Determination of meropenem in bacterial media by LC-MS/MS.
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DOI：
10.1016/j.jchromb.2014.05.002
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发表时间：
2014-06-15
影响因子：
3
通讯作者：
Yang, Katherine
中科院分区：
文献类型：
--
作者：
Huang, Liusheng;Haagensen, Janus;Verotta, Davide;Lizak, Patricia;Aweeka, Francesca;Yang, Katherine
为了支持生物膜介导感染的动态体外人体药代动力学/药效动力学模拟模型的开发并研究美罗培南的稳定性，开发了一种用于测定 Luria Bertani (LB) 培养基中美罗培南的 LC-MS/MS 方法，并在 API2000 LC-MS/MS 系统中进行了验证。还在 M9 培养基中进行了部分验证。将 100 μL（或 M9 培养基为 25 μL）的样品等分试样与内标 (IS) 头孢他啶混合并过滤。将滤液直接注入C8柱上，用甲酸铵(10mM，pH4)和乙腈(0.1％甲酸)以梯度方式洗脱。使用 ESI+ 和 MRM 进行定量，美罗培南的离子对 m/z 384 → 68，IS 的离子对 m/z 547 → 468。校准曲线浓度范围为 50 至 25,000 ng/mL。回收率超过98%。与流动相溶剂相比，在 LB 介质中，在整个检测期间观察到显着的信号抑制，但基质效应通过 IS 得到了很好的补偿。在 M9 培养基中，观察到的信号抑制要少得多。该方法简单、快速、可靠。利用该方法测试了美罗培南在LB和M9培养基中的稳定性。在 LB 培养基 (37 °C) 和 M9 培养基 (30 °C) 中至少 8 小时内未观察到明显降解，但过夜（约 20 小时）观察到超过 15% 的降解。该方法转移至 API5000 LC-MS/MS 系统，使用美罗培南-d6 作为内标。
To support the development of a dynamic in vitro human Pharmacokinetic/Pharmacodynamic simulation model for biofilm-mediated infections and study stability of meropenem, an LC-MS/MS method for the determination of meropenem in Luria Bertani (LB) media was developed and validated in an API2000 LC-MS/MS system. A partial validation was also performed in M9 media. Sample aliquots of 100 μL (or 25 μL for M9 media) were mixed with the internal standard (IS) ceftazidime and filtered. The filtrate was directly injected onto a C8 column eluted with ammonium formate (10mM, pH4) and acetonitrile (0.1% formic acid) in a gradient mode. ESI+ and MRM with ion pair m/z 384 → 68 for meropenem and m/z 547 → 468 for the IS were used for quantification. The calibration curve concentration range was 50 to 25,000 ng/mL. The recovery was over 98%. In LB media, significant signal suppression was observed throughout the time period of detection when compared with mobile phase solvents, but the matrix effect was compensated well with the IS. In M9 media, much less signal suppression was observed. The method is simple, fast, and reliable. Using the method, stability of meropenem in LB and M9 media were tested. No significant degradation was observed for at least 8 hours in both LB media (37 °C) and M9 media (30 °C), but more than 15% degradation was observed overnight (∼20hr). The method was transferred to an API5000 LC-MS/MS system using meropenem-d6 as the IS.
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DOI：
10.1016/j.jchromb.2007.05.043
发表时间：
2007-09-01
影响因子：
3
作者：
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发表时间：
2011-05-01
影响因子：
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作者：
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