Chemical Composition and Immunomodulatory Activity of Essential Oils from Rhododendron albiflorum.
Chemical Composition and Immunomodulatory Activity of Essential Oils from Rhododendron albiflorum.
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DOI：
10.3390/molecules26123652
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发表时间：
2021-06-15
期刊：
影响因子：
--
通讯作者：
Quinn MT
中科院分区：
文献类型：
--
作者：
Schepetkin IA;Özek G;Özek T;Kirpotina LN;Khlebnikov AI;Quinn MT
杜鹃花（杜鹃花科）提取物含有类黄酮，色酮，萜类化合物，类固醇和精油，并用于传统的民族植物药。然而，从这些植物中分离的精油的免疫调节活性知之甚少。因此，我们从玫瑰的花和叶中分离出精油。albiflorum（级联杜鹃花），并分析了它们的化学组成和先天免疫调节活性。花（REOF 1）与叶（REOLv）精油的组成分析揭示了显著差异。REOF 1主要由单萜（92%）组成，而倍半萜以相对低的量被发现。与此相反，REOLv主要由倍半萜（90.9%），与少量的单萜。REOLv及其主要的倍半萜（viridiflorol，spathulenol，curzerene和germacrone）诱导人中性粒细胞，C20小胶质细胞和用N-甲酰肽受体1（FPR 1）或FPR 2转染的HL 60细胞中的细胞内Ca 2+动员。另一方面，用这些精油或组分化合物预处理抑制激动剂诱导的人中性粒细胞中的Ca 2+动员和趋化性以及激动剂诱导的小胶质细胞和FPR转染的HL 60细胞中的Ca 2+动员，表明这些化合物对[Ca 2 +]i的直接作用使细胞对随后的激动剂活化脱敏。反向药效团映射建议这些化合物的几个潜在的激酶靶点，然而，这些目标不支持激酶结合试验。我们的研究结果提供了细胞和分子基础，以解释至少部分的有益免疫特性的R。albiflorum精油的研究表明，这种植物叶子的精油可能有效地调节一些先天免疫反应，可能是通过抑制中性粒细胞迁移。
Rhododendron (Ericaceae) extracts contain flavonoids, chromones, terpenoids, steroids, and essential oils and are used in traditional ethnobotanical medicine. However, little is known about the immunomodulatory activity of essential oils isolated from these plants. Thus, we isolated essential oils from the flowers and leaves of R. albiflorum (cascade azalea) and analyzed their chemical composition and innate immunomodulatory activity. Compositional analysis of flower (REOFl) versus leaf (REOLv) essential oils revealed significant differences. REOFl was comprised mainly of monoterpenes (92%), whereas sesquiterpenes were found in relatively low amounts. In contrast, REOLv was primarily composed of sesquiterpenes (90.9%), with a small number of monoterpenes. REOLv and its primary sesquiterpenes (viridiflorol, spathulenol, curzerene, and germacrone) induced intracellular Ca2+ mobilization in human neutrophils, C20 microglial cells, and HL60 cells transfected with N-formyl peptide receptor 1 (FPR1) or FPR2. On the other hand, pretreatment with these essential oils or component compounds inhibited agonist-induced Ca2+ mobilization and chemotaxis in human neutrophils and agonist-induced Ca2+ mobilization in microglial cells and FPR-transfected HL60 cells, indicating that the direct effect of these compounds on [Ca2+]i desensitized the cells to subsequent agonist activation. Reverse pharmacophore mapping suggested several potential kinase targets for these compounds; however, these targets were not supported by kinase binding assays. Our results provide a cellular and molecular basis to explain at least part of the beneficial immunotherapeutic properties of the R. albiflorum essential oils and suggest that essential oils from leaves of this plant may be effective in modulating some innate immune responses, possibly by inhibition of neutrophil migration.
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DOI：
10.3390/molecules23092239
发表时间：
2018-09-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Gautam V;Kohli SK;Arora S;Bhardwaj R;Kazi M;Ahmad A;Raish M;Ganaie MA;Ahmad P
通讯作者：
Ahmad P
影响因子：
3.2
作者：
Garcia-Mesa, Yoelvis;Jay, Taylor R.;Alvarez-Carbonell, David
通讯作者：
Alvarez-Carbonell, David
影响因子：
5.4
作者：
Abu-Darwish, M. S.;Cabral, C.;Salgueiro, L.
通讯作者：
Salgueiro, L.
影响因子：
4.4
作者：
Gautam, Vandana;Sharma, Anket;Ahmad, Parvaiz
通讯作者：
Ahmad, Parvaiz
影响因子：
4.6
作者：
Daina A;Michielin O;Zoete V
通讯作者：
Zoete V