Identification and characterization of microRNAs in tree peony during chilling induced dormancy release by high-throughput sequencing.
Identification and characterization of microRNAs in tree peony during chilling induced dormancy release by high-throughput sequencing.
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高通量测序对牡丹低温诱导休眠释放过程中 microRNA 的鉴定和表征
DOI：
10.1038/s41598-018-22415-5
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发表时间：
2018-03-14
影响因子：
4.6
通讯作者：
Gai S
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang Y;Gao X;Liu C;Gai S
牡丹是世界上最有价值的园艺和药用植物之一，必须经历冬季才能打破休眠。从分子水平对牡丹休眠解除过程的研究已有报道，但对牡丹休眠解除过程中miRNA调控的研究尚不充分。在这项研究中，高通量测序鉴定和表征的miRNAs在三个文库（6天，18天和24天的冷冻处理）。结果表明，在所有的miRNA中，有7,122个、10,076个和9,097个独特的miRNA序列，分别属于52个、87个和68个miRNA家族。在休眠解除过程中共鉴定出32个保守的miRNAs和17个新的miRNAs。在62个miRNA家族中有771个单一基因可作为潜在靶基因。结果表明，共获得112个差异表达的miRNAs，其中55个miRNAs在3个文库中共有，28个miRNAs仅在18 d冷时文库中发现。RT-qPCR验证了15种保守miRNA的表达模式，并将其分为4种类型。结合我们的微阵列数据，在相同的处理条件下，5个miRNAs（miR 156 k，miR 159 a，miR 167 a，miR 169 a和miR 172 a）与其靶基因的表达呈负相关。本研究结果为牡丹休眠解除提供了新的分子基础。
Tree peony, one of the most valuable horticultural and medicinal plants in the world, has to go through winter to break dormancy. Growing studies from molecular aspects on dormancy release process have been reported, but inadequate study has been done on miRNA-guided regulation in tree peony. In this study, high-throughput sequencing was employed to identify and characterize miRNAs in three libraries (6 d, 18 d and 24 d chilling treatments). There were 7,122, 10,076 and 9,097 unique miRNA sequences belonging to 52, 87 and 68 miRNA families, respectively. A total of 32 conserved miRNAs and 17 putative novel miRNAs were identified during dormancy release. There were 771 unigenes as potential targets of 62 miRNA families. Total 112 known miRNAs were differentially expressed, of which 55 miRNAs were shared among three libraries and 28 miRNAs were only found in 18 d chilling duration library. The expression patterns of 15 conserved miRNAs were validated and classified into four types by RT-qPCR. Combining with our microarray data under same treatments, five miRNAs (miR156k, miR159a, miR167a, miR169a and miR172a) were inversely correlated to those of their target genes. Our results would provide new molecular basis about dormancy release in tree peony.
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