Phorbol 12‐myristate 13‐acetate (PMA) increases the expression of the nerve growth factor (NGF) gene in mouse L‐929 fibroblasts
Phorbol 12‐myristate 13‐acetate (PMA) increases the expression of the nerve growth factor (NGF) gene in mouse L‐929 fibroblasts
复制标题
佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 增加小鼠 L-929 成纤维细胞中神经生长因子 (NGF) 基因的表达
DOI：
10.1016/0014-5793(90)80149-d
复制
发表时间：
1990
期刊：
影响因子：
3.5
通讯作者：
P. Brachet
中科院分区：
文献类型：
--
作者：
D. Wion;Donal Mac Grogan;R. Houlgatte;P. Brachet
5μM K-252a是一种抑制几种蛋白激酶活性的化合物，可阻止L-929成纤维细胞暴露于血清后NGF信使核糖核酸池的增加。为了进一步研究这一现象，将生长在无血清介质中的L细胞暴露于环核苷酸类似物、二价阳离子载体或佛波酯PMA。只有后一种化合物诱导了NGF mRNA池的增强，这表明蛋白激酶C参与了NGF转录本的上调。PMA或血清的作用还需要蛋白质的合成，因为在环己亚胺存在下，NGF转录本的水平保持稳定。
The rise of the NGF mRNA pool which takes place following exposure of L-929 fibroblasts to serum was prevented in the presence of 5 μM K-252a, a compound which inhibits several species of protein kinase activities. To characterize further this phenomenon, L-929 cells growing in a serum-free medium were exposed to cyclic nucleotide analogs, to a divalent cation ionophore or to the phorbol ester PMA. Only this latter compound induced an enhancement of the NGF mRNA pool, suggesting an involvement of protein kinase C in the upregulation of the NGF transcripts. The effects of PMA or serum also require a synthesis of protein since the level of NGF transcripts remained stable in the presence of cycloheximide.
星形细胞瘤细胞中β-肾上腺素能受体激活调节神经生长因子生物合成：c-Fos 蛋白的潜在作用。
DOI：
10.1073/pnas.86.10.3891
发表时间：
1989
影响因子：
11.1
作者：
Mocchetti,I;DeBernardi,MA;Szekely,AM;Alho,H;Brooker,G;Costa,E
通讯作者：
Costa,E
影响因子：
56.9
作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt
通讯作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt